College of Agronomy, Gansu Agricultural University, Lanzhou, 730070, PR China; Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, 730070, PR China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, PR China.
Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, 730070, PR China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, PR China.
Plant Physiol Biochem. 2020 Nov;156:167-177. doi: 10.1016/j.plaphy.2020.09.012. Epub 2020 Sep 14.
Mitogen-activated protein kinase 3 (MAPK3) is involved in plant growth and development, as well as response to adverse stress. Here we aimed to explore the role of StMAPK3 in response to salt and osmosis stress. Polyethylene glycol (PEG) (5% and 10%) and mannitol (40 mM and 80 mM) were used to induce osmosis stress. To induce salinity stress, potato plant was cultured with NaCl (40 mM and 80 mM). StMAPK3 overexpression and RNA interference-mediated StMAPK3 knockdown were constructed to explore the role of StMAPK3 in potato growth, stomatal aperture size, activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), and contents of HO, proline and malonaldehyde (MDA). Meanwhile, we detected transpiration, net photosynthesis, stomatal conductance, and water use efficiency. Subcellular location of StMAPK3 protein was also detected. PEG, mannitol and NaCl treatments induced the accumulation of StMAPK3 mRNA in potato plants. StMAPK3 protein was located on the membrane and nucleus. Abnormal expression of StMAPK3 changed potato phenotypes, enzyme activity of SOD, CAT and POD, as well as HO, proline and MDA contents under osmosis and salinity stress. Photosynthesis and stomatal aperture were regulated by StMAPK3 in potato treated by PEG, mannitol and NaCl. Modulation of potato phenotypes and physiological activity indicates StMAPK3 as a regulator of osmosis and salinity tolerance.
丝裂原活化蛋白激酶 3（MAPK3）参与植物的生长发育以及对不利胁迫的响应。在这里，我们旨在探索 StMAPK3 在应对盐和渗透胁迫中的作用。使用聚乙二醇（PEG）（5%和 10%）和甘露醇（40 mM 和 80 mM）诱导渗透胁迫。为了诱导盐胁迫，用 NaCl（40 mM 和 80 mM）培养马铃薯植株。构建了 StMAPK3 的过表达和 RNA 干扰介导的 StMAPK3 敲低，以探讨 StMAPK3 在马铃薯生长、气孔孔径大小、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性以及 HO、脯氨酸和丙二醛（MDA）含量以及蒸腾作用、净光合作用、气孔导度和水分利用效率方面的作用。同时，我们检测了 transpiration、net photosynthesis、stomatal conductance 和 water use efficiency。还检测了 StMAPK3 蛋白的亚细胞定位。PEG、甘露醇和 NaCl 处理诱导马铃薯植株中 StMAPK3 mRNA 的积累。StMAPK3 蛋白位于膜和核上。StMAPK3 异常表达改变了渗透胁迫和盐胁迫下马铃薯的表型、SOD、CAT 和 POD 的酶活性以及 HO、脯氨酸和 MDA 的含量。PEG、甘露醇和 NaCl 处理的马铃薯中，StMAPK3 调节光合作用和气孔开度。马铃薯表型和生理活性的调节表明 StMAPK3 是渗透和盐胁迫耐受性的调节剂。
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