State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.
College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Int J Mol Sci. 2024 Mar 25;25(7):3662. doi: 10.3390/ijms25073662.
MAPKKs, as one of the main members of the mitogen-activated protein kinase (MAPK) cascade pathway, are located in the middle of the cascade and are involved in many physiological processes of plant growth and development, as well as stress tolerance. Previous studies have found that is responsive to drought and salt stress. To further investigate the function and regulatory mechanism of in potato stress response, potato variety 'Atlantic' was subjected to drought and NaCl treatments, and the expression of the gene was detected by qRT-PCR. overexpression and RNA interference-mediated knockdown potato plants were constructed. The relative water content, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities, as well as proline (Pro) and malondialdehyde (MDA) contents of plant leaves, were also assayed under drought and NaCl stress. The StMAPKK5 interacting proteins were identified and validated by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC). The results showed that the expression of was significantly up-regulated under drought and NaCl stress conditions. The StMAPKK5 protein was localized in the nucleus, cytoplasm, and cell membrane. The expression of affected the relative water content, the enzymatic activities of SOD, CAT, and POD, and the proline and MDA contents of potatoes under drought and salt stress conditions. These results suggest that plays a significant role in regulating drought and salt tolerance in potato crop. Yeast two-hybrid (Y2H) screening identified four interacting proteins: StMYB19, StZFP8, StPUB-like, and StSKIP19. BiFC confirmed the authenticity of the interactions. These findings suggest that is crucial for potato growth, development, and response to adversity.
MAPKKs，作为丝裂原活化蛋白激酶（MAPK）级联途径的主要成员之一，位于级联的中间，参与植物生长发育和胁迫耐受的许多生理过程。先前的研究发现， 对干旱和盐胁迫有响应。为了进一步研究 基因在马铃薯胁迫响应中的功能和调控机制，对马铃薯品种‘Atlantic’进行干旱和 NaCl 处理，并通过 qRT-PCR 检测 基因的表达。构建了 过表达和 RNA 干扰介导的 基因敲低马铃薯植株。在干旱和 NaCl 胁迫下，还测定了植株叶片的相对含水量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性以及脯氨酸（Pro）和丙二醛（MDA）含量。通过酵母双杂交（Y2H）和双分子荧光互补（BiFC）鉴定和验证了 StMAPKK5 的互作蛋白。结果表明，在干旱和 NaCl 胁迫条件下， 基因的表达显著上调。StMAPKK5 蛋白定位于细胞核、细胞质和细胞膜。 基因的表达影响干旱和盐胁迫下马铃薯的相对含水量、SOD、CAT 和 POD 的酶活性以及脯氨酸和 MDA 的含量。这些结果表明， 在调节马铃薯的抗旱耐盐性方面发挥着重要作用。酵母双杂交（Y2H）筛选鉴定了四个互作蛋白：StMYB19、StZFP8、StPUB-like 和 StSKIP19。BiFC 证实了互作的真实性。这些发现表明 对于马铃薯的生长、发育和应对逆境至关重要。
