Kimura Angela Hitomi, Dahmer Débora, Isawa Luana Ayumi, da Silva Ana Beatriz Olivetti, Souza Lucas Marcelino Dos Santos, Takata Pedro Henrique, Scandorieiro Sara, Deonas Anastácia Nikolaos, Germiniani-Cardozo Jennifer, Vespero Eliana Carolina, Perugini Marcia Regina Eches, Lincopan Nilton, Garcia Lonni Audrey Alesandra Stinghen, Nakazato Gerson, Kobayashi Renata Katsuko Takayama
Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina 86057-970, Brazil.
Department of Biochemistry and Biotechnology, State University of Londrina, Londrina 86057-970, Brazil.
Pharmaceutics. 2025 Apr 10;17(4):503. doi: 10.3390/pharmaceutics17040503.
Wounds from burns are susceptible to infections, allowing multidrug-resistant microorganisms to complicate treatments and patient recovery. This highlights the development of new strategies to control these microorganisms. This work evaluated the antibacterial activity of hydrogels containing biogenic silver nanoparticles (bio-AgNP) and essential oil (OEO) against multidrug-resistant bacteria. The formulations were subjected to organoleptic, pharmacotechnical, and stability characterization and antimicrobial activity assessment by time-kill tests and alternative methods, an ex vivo model using porcine skin, and an in vivo model using . All hydrogels maintained their stability after the thermal stress. The hydrogel containing bio-AgNP + OEO 1% (HAgNP + OEO1) presented bactericidal effectiveness, within 2 h, against both Gram-positive and Gram-negative multidrug-resistant bacteria in the time-kill test. For alternative testing, HAgNP + OEO1 was compared with 1% silver sulfadiazine (SS) and the base formulation. In the ex vivo test, both HAgNP + OEO1 and SS treatments showed a similar reduction in superficial washing of the burn for 999, while for , the reduction was more expressive for SS treatment. In the burn tissue, HAgNP + OEO1 treatment was more effective against 999, while for 1461, both formulations were similarly effective. In the test, survival rates after 48 h were 84% for the control group (base) and 50% for both HAgNP + OEO1 and SS treatment groups. This study demonstrates that the hydrogel combining antimicrobials is effective against multidrug-resistant microorganisms, offering a promising alternative for the treatment of infected burns.
烧伤伤口易受感染，使得耐多药微生物会让治疗和患者康复变得复杂。这凸显了开发控制这些微生物的新策略的必要性。本研究评估了含有生物源银纳米颗粒（bio-AgNP）和精油（OEO）的水凝胶对耐多药细菌的抗菌活性。通过感官、药剂学和稳定性表征以及通过时间杀菌试验和替代方法、使用猪皮的离体模型和使用[此处缺失具体内容]的体内模型对制剂进行了抗菌活性评估。所有水凝胶在热应激后均保持其稳定性。含有1% bio-AgNP + OEO的水凝胶（HAgNP + OEO1）在时间杀菌试验中，2小时内对革兰氏阳性和革兰氏阴性耐多药细菌均呈现杀菌效果。在替代试验中，将HAgNP + OEO1与1%磺胺嘧啶银（SS）和基础制剂进行了比较。在离体试验中，HAgNP + OEO1和SS处理在烧伤创面浅层冲洗方面均显示出类似的999减少情况，而对于[此处缺失具体内容]，SS处理的减少更为明显。在烧伤组织中，HAgNP + OEO1处理对999更有效，而对于1461，两种制剂效果相似。在[此处缺失具体内容]试验中，对照组（基础组）48小时后的存活率为84%，HAgNP + OEO1和SS处理组均为50%。本研究表明，组合抗菌剂的水凝胶对耐多药微生物有效，为治疗感染性烧伤提供了一种有前景的替代方法。
