Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, Hunan, China; Key Lab of Non-wood Forest Products of State Forestry Administration, College of Forestry, Central South University of Forestry and Technology, Changsha 410004, Hunan, China.
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, Hunan, China; Key Lab of Non-wood Forest Products of State Forestry Administration, College of Forestry, Central South University of Forestry and Technology, Changsha 410004, Hunan, China.
Int J Biol Macromol. 2020 Nov 15;163:1759-1767. doi: 10.1016/j.ijbiomac.2020.09.106. Epub 2020 Sep 20.
Leucine-rich repeat-containing genes (LRRs) have been reported to play important roles in responses to diseases. However, we poorly understood the response of LRRs to Fusarium wilt infection in tung tree (Vernicia fordii), which is an important economic tree. Here, 437 LRR-containing proteins containing nine types of LRR domains were identified in V. fordii genome. Phylogenetic analysis suggested that nine types of LRR domains could not be divided into separate classes, implying that these LRR domains had a common origin. Totally, 27 LRRs were related to possible resistance to Fusarium wilt after 2, 8, and 13 days post-inoculation. We further found that Vf06G1605 was up-regulate under Fusarium wilt infection after these three time points, Vf10G1602 and Vf02G1413 were up-regulated at 8, and 13 dpi, while Vf07G2320 was down-regulated at these three time points. The WGCNA and promoter elements suggested that WRKY possibly regulate the responses of LRRs to Fusarium wilt infection. This study highlighted the phylogeny and function of LRRs in V. fordii and provided a systematic analysis of these genes in the V. fordii genome. Our results presented here might clearly illustrate physiological mechanisms of resistance to Fusarium wilt infection and the target of marker-assisted breeding in V. fordii.
富含亮氨酸重复序列的基因 (LRRs) 在应对疾病方面发挥着重要作用。然而，我们对油桐（Vernicia fordii）中 LRR 对枯萎病感染的反应知之甚少，油桐是一种重要的经济树种。在这里，在 V. fordii 基因组中鉴定出 437 种含有九种 LRR 结构域的 LRR 蛋白。系统发育分析表明，九种 LRR 结构域不能分为单独的类群，这意味着这些 LRR 结构域具有共同的起源。共有 27 个 LRR 与可能对枯萎病的抗性有关，在接种后 2、8 和 13 天。我们进一步发现，Vf06G1605 在这三个时间点后在枯萎病感染下上调，Vf10G1602 和 Vf02G1413 在 8 和 13 dpi 上调，而 Vf07G2320 在这三个时间点下调。WGCNA 和启动子元件表明 WRKY 可能调节 LRR 对枯萎病感染的反应。本研究强调了 LRR 在 V. fordii 中的系统发育和功能，并对 V. fordii 基因组中的这些基因进行了系统分析。我们这里提出的结果可能清楚地说明了对枯萎病感染的抗性的生理机制和 V. fordii 中标记辅助选择的目标。
