猪带绦虫 3 个新重组抗原的鉴定和评估用于囊尾蚴病的免疫诊断。
Characterization and evaluation of three new recombinant antigens of Taenia solium for the immunodiagnosis of cysticercosis.
机构信息
Instituto de Investigaciones Biomédicas "Dr. Francisco J. Triana Alonso" (BIOMED), Facultad de Ciencias de la Salud, Universidad de Carabobo Sede Aragua, Maracay, Venezuela.
Instituto de Salud Carlos III, Centro Nacional de Microbiología, 28220, Majadahonda, Madrid, Spain.
出版信息
Mol Biochem Parasitol. 2020 Nov;240:111321. doi: 10.1016/j.molbiopara.2020.111321. Epub 2020 Sep 19.
Cysticerci of Taenia solium cause cysticercosis, with neurocysticercosis (NCC) as the major pathology. Sensible and specific recombinant antigens would be an source of antigen for immunodiagnosis. The objective of this work was the molecular characterization and evaluation, of three news recombinant antigens (TsF78, TsP43 and TsC28), obtained by screening of a Taenia solium cDNA library. The three cDNA were analysed by bioinformatic programs, subcloned and expresed. The purified proteins were evaluated in ELISA using cyst fluid as control. TsF78 is filamina, TsP43 a peroxidase and TsC28 collagen XV. The sensitivity and specificity of the recombinant proteins were; TsF78 93.8 % and 95.0 %, TsP62 91.7 % and 93.3 %, TsC28 85.4 % and 93.3 %, respectively, while the cyst fluid showed a sensitivity of 87.5 % and a specificity of 76.7 %. Given its high sensitivity and specificity, the recombinant proteins TsF78 and TsP62 could be used in the diagnosis of cysticercosis.
猪带绦虫的囊尾蚴引起囊尾蚴病，其中神经囊尾蚴病（NCC）是主要的病理学表现。敏感和特异的重组抗原将成为免疫诊断的抗原来源。本工作的目的是通过筛选猪带绦虫 cDNA 文库，对三个新的重组抗原（TsF78、TsP43 和 TsC28）进行分子特征分析和评估。通过生物信息学程序对三个 cDNA 进行分析、亚克隆和表达。使用囊液作为对照，在 ELISA 中评估纯化的蛋白质。TsF78 是纤连蛋白，TsP43 是过氧化物酶，TsC28 是胶原 XV。重组蛋白的敏感性和特异性分别为：TsF78 为 93.8%和 95.0%，TsP62 为 91.7%和 93.3%，TsC28 为 85.4%和 93.3%，而囊液的敏感性为 87.5%，特异性为 76.7%。鉴于其高敏感性和特异性，重组蛋白 TsF78 和 TsP62 可用于囊尾蚴病的诊断。
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