Wilczynski Milosz, Kielbik Michal, Senderowska Daria, Krawczyk Tomasz, Szymanska Bozena, Klink Magdalena, Bieńkiewicz Jan, Romanowicz Hanna, Frühauf Filip, Malinowski Andrzej
Department of Operative Gynecology, Endoscopy and Gynecologic Oncology, Polish Mother's Memorial Hospital Research Institute, 281 Rzgowska Str., 93-338 Lodz, Poland.
Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa Str., 93-232 Lodz, Poland.
Cancers (Basel). 2020 Sep 19;12(9):2680. doi: 10.3390/cancers12092680.
High levels of miRNA-103/107 are associated with poor outcomes in the case of breast cancer patients. MiRNA-103/107-DICER axis may be one of the key regulators of cancer aggressiveness. MiRNA-103/107 expression levels have never been related to patients' clinicopathological data in epithelial ovarian cancer. We aimed to assess miRNA-103/107 expression levels in high grade serous ovarian cancer tissues. Expression levels of both miRNAs were related to the clinicopathological features and survival. We also evaluated expression levels of miRNA-103/107 and DICER in selected ovarian cancer cell lines (A2780, A2780cis, SK-OV-3, OVCAR3). We assessed the relative expression of miRNA-103/107 (quantitative reverse transcription-polymerase chain reaction) in fifty archival formalin-fixed paraffin-embedded tissue samples of primary high grade serous ovarian cancer. Then, miRNA-103/107 and DICER expression levels were evaluated in selected ovarian cancer cell lines. Additionally, DICER, N-/E-cadherin protein levels were assessed with the use of western blot. We identified miRNA-107 up-regulation in ovarian cancer in comparison to healthy tissues ( = 0.0005). In the case of miRNA-103, we did not observe statistically significant differences between cancerous and healthy tissues ( = 0.07). We did not find any correlations between miRNA-103/107 expression levels and clinicopathological features. Kaplan-Meier survival (disease-free and overall survival) analysis revealed that both miRNAs could not be considered as prognostic factors. SK-OV-3 cancer cell lines were characterized by high expression of miRNA-103/107, relatively low expression of DICER (western-blot), and relatively high N-cadherin levels in comparison to other ovarian cancer cell lines. Clinical and prognostic significance of miRNA-103/107 was not confirmed in our study.
在乳腺癌患者中，高水平的miRNA - 103/107与不良预后相关。miRNA - 103/107 - DICER轴可能是癌症侵袭性的关键调节因子之一。在上皮性卵巢癌中，miRNA - 103/107的表达水平从未与患者的临床病理数据相关联。我们旨在评估高级别浆液性卵巢癌组织中miRNA - 103/107的表达水平。这两种miRNA的表达水平与临床病理特征及生存率相关。我们还评估了所选卵巢癌细胞系（A2780、A2780cis、SK - OV - 3、OVCAR3）中miRNA - 103/107和DICER的表达水平。我们评估了50例原发性高级别浆液性卵巢癌存档福尔马林固定石蜡包埋组织样本中miRNA - 103/107的相对表达（定量逆转录 - 聚合酶链反应）。然后，在所选卵巢癌细胞系中评估miRNA - 103/107和DICER的表达水平。此外，使用蛋白质印迹法评估DICER、N - /E - 钙黏蛋白的蛋白水平。与健康组织相比，我们发现卵巢癌中miRNA - 107上调（P = 0.0005）。对于miRNA - 103，我们未观察到癌组织与健康组织之间存在统计学显著差异（P = 0.07）。我们未发现miRNA - 103/107表达水平与临床病理特征之间存在任何相关性。Kaplan - Meier生存分析（无病生存期和总生存期）显示，这两种miRNA均不能被视为预后因素。与其他卵巢癌细胞系相比，SK - OV - 3癌细胞系的特征是miRNA - 103/107高表达、DICER相对低表达（蛋白质印迹法）以及N - 钙黏蛋白水平相对较高。在我们的研究中，未证实miRNA - 103/107的临床及预后意义。
