Yazdani-Ahmadabadi Hossein, Yu Kai, Khoddami Sara, F Felix Demian, Yeh Han H, Luo Haiming D, Moskalev Igor, Wang Qiong, Wang Rizhi, Grecov Dana, Fazli Ladan, Lange Dirk, Kizhakkedathu Jayachandran N
Department of Chemistry, University of British Columbia, Vancouver V6T 1Z3, British Columbia, Canada.
Centre for Blood Research, Life Science Institute, University of British Columbia, Vancouver V6T 1Z3, British Columbia, Canada.
ACS Nanosci Au. 2022 Oct 28;3(1):67-83. doi: 10.1021/acsnanoscienceau.2c00040. eCollection 2023 Feb 15.
A major medical device-associated complication is the biofilm-related infection post-implantation. One promising approach to prevent this is to coat already commercialized medical devices with effective antibiofilm materials. However, developing a robust high-performance antibiofilm coating on devices with a nonflat geometry remains unmet. Here, we report the development of a facile scalable nanoparticle-based antibiofilm silver composite coating with long-term activity applicable to virtually any objects including difficult-to-coat commercially available medical devices utilizing a catecholic organic-aqueous mixture. Using a screening approach, we have identified a combination of the organic-aqueous buffer mixture which alters polycatecholamine synthesis, nanoparticle formation, and stabilization, resulting in controlled deposition of in situ formed composite silver nanoparticles in the presence of an ultra-high-molecular-weight hydrophilic polymer on diverse objects irrespective of its geometry and chemistry. Methanol-mediated synthesis of polymer-silver composite nanoparticles resulted in a biocompatible lubricious coating with high mechanical durability, long-term silver release (∼90 days), complete inhibition of bacterial adhesion, and excellent killing activity against a diverse range of bacteria over the long term. Coated catheters retained their excellent activity even after exposure to harsh mechanical challenges (rubbing, twisting, and stretching) and storage conditions (>3 months stirring in water). We confirmed its excellent bacteria-killing efficacy (>99.999%) against difficult-to-kill bacteria () and high biocompatibility using percutaneous catheter infection mice and subcutaneous implant rat models, respectively, . The developed coating approach opens a new avenue to transform clinically used medical devices (e.g., urinary catheters) to highly infection-resistant devices to prevent and treat implant/device-associated infections.
一种主要的与医疗设备相关的并发症是植入后与生物膜相关的感染。一种有前景的预防方法是用有效的抗生物膜材料涂覆已商业化的医疗设备。然而，在具有非平面几何形状的设备上开发一种坚固的高性能抗生物膜涂层仍然是未满足的需求。在此，我们报告了一种基于纳米颗粒的简便可扩展抗生物膜银复合涂层的开发，该涂层具有长期活性，适用于几乎任何物体，包括利用儿茶酚有机 - 水混合物难以涂覆的市售医疗设备。通过筛选方法，我们确定了一种有机 - 水缓冲混合物的组合，该组合改变了聚儿茶酚胺的合成、纳米颗粒的形成和稳定性，从而在超高分子量亲水性聚合物存在下，使原位形成的复合银纳米颗粒在各种物体上可控沉积，而不论其几何形状和化学性质如何。甲醇介导的聚合物 - 银复合纳米颗粒的合成产生了一种具有高机械耐久性、长期银释放（约90天）、完全抑制细菌粘附以及对多种细菌具有长期优异杀灭活性的生物相容性润滑涂层。涂覆的导管即使在经受苛刻的机械挑战（摩擦、扭曲和拉伸）以及储存条件（在水中搅拌>3个月）后仍保持其优异的活性。我们分别使用经皮导管感染小鼠和皮下植入大鼠模型证实了其对难杀灭细菌的优异杀菌效果（>99.999%）和高生物相容性。所开发的涂覆方法为将临床使用的医疗设备（如导尿管）转变为高度抗感染的设备以预防和治疗植入物/设备相关感染开辟了一条新途径。
