García-Villén Fátima, Sánchez-Espejo Rita, Borrego-Sánchez Ana, Cerezo Pilar, Cucca Lucia, Sandri Giuseppina, Viseras César
Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Campus of Cartuja, 18071 Granada, Spain.
Andalusian Institute of Earth Sciences, CSIC-UGR (Consejo Superior de Investigaciones Científicas-Universidad de Granada), Avenida de las Palmeras 4, Armilla, 18100 Granada, Spain.
Pharmaceutics. 2020 Sep 18;12(9):891. doi: 10.3390/pharmaceutics12090891.
Inorganic hydrogels formulated with spring waters and clay minerals are used to treat musculoskeletal disorders and skin affections. Their underlying mechanism of action for skin disorders is not clear, although it is usually ascribed to the chemical composition of the formulation. The aim of this study was to assess the composition and in vitro release of elements with potential wound healing effects from hydrogels prepared with two nanoclays and natural spring water. In vitro Franz cell studies were used and the element concentration was measured by inductively coupled plasma techniques. Biocompatibility studies were used to evaluate the potential toxicity of the formulation against fibroblasts. The studied hydrogels released elements with known therapeutic interest in wound healing. The released ratios of some elements, such as Mg:Ca or Zn:Ca, played a significant role in the final therapeutic activity of the formulation. In particular, the proliferative activity of fibroblasts was ascribed to the release of Mn and the Zn:Ca ratio. Moreover, the importance of formulative studies is highlighted, since it is the optimal combination of the correct ingredients that makes a formulation effective.
由泉水和粘土矿物配制而成的无机水凝胶被用于治疗肌肉骨骼疾病和皮肤疾病。尽管其对皮肤疾病的潜在作用机制通常归因于配方的化学成分，但其确切机制尚不清楚。本研究的目的是评估由两种纳米粘土和天然泉水制备的水凝胶中具有潜在伤口愈合作用的元素组成及其体外释放情况。采用体外弗兰兹扩散池研究，并通过电感耦合等离子体技术测量元素浓度。通过生物相容性研究评估该配方对成纤维细胞的潜在毒性。所研究的水凝胶释放出对伤口愈合具有已知治疗意义的元素。某些元素的释放比例，如镁：钙或锌：钙，在配方的最终治疗活性中发挥了重要作用。特别是，成纤维细胞的增殖活性归因于锰的释放和锌：钙比例。此外，强调了配方研究的重要性，因为正是正确成分的最佳组合才使配方有效。
