Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, 76100, Rehovot, Israel.
Laboratory of Enzymology, Department of Cellular Biology, Biological Sciences Institute, University of Brasilia, Brasilia, DF, 70910-900, Brazil.
ISME J. 2021 Feb;15(2):409-420. doi: 10.1038/s41396-020-00767-z. Epub 2020 Sep 22.
The phyllosphere - the aerial parts of plants - is an important microbial habitat that is home to diverse microbial communities. The spatial organization of bacterial cells on leaf surfaces is non-random, and correlates with leaf microscopic features. Yet, the role of microscale interactions between bacterial cells therein is not well understood. Here, we ask how interactions between immigrant bacteria and resident microbiota affect the spatial organization of the combined community. By means of live imaging in a simplified in vitro system, we studied the spatial organization, at the micrometer scale, of the biocontrol agent Pseudomonas fluorescens A506 and the plant pathogen P. syringae B728a when introduced to pear and bean leaf microbiota (the corresponding native plants of these strains). We found significant co-localization of immigrant and resident microbial cells at distances of a few micrometers, for both strains. Interestingly, this co-localization was in part due to preferential attachment of microbiota cells near newly formed P. fluorescens aggregates. Our results indicate that two-way immigrant bacteria - resident microbiota interactions affect the microscale spatial organization of leaf microbiota, and possibly that of other surface-related microbial communities.
叶际 - 植物的气生部分 - 是一个重要的微生物栖息地，拥有多样的微生物群落。叶片表面细菌细胞的空间组织是非随机的，并与叶片微观特征相关。然而，细菌细胞之间的微观相互作用的作用尚不清楚。在这里，我们研究了外来细菌和常驻微生物群落之间的相互作用如何影响组合群落的空间组织。通过简化的体外系统中的实时成像，我们研究了当生物防治剂荧光假单胞菌 A506 和植物病原菌丁香假单胞菌 B728a 引入到梨和豆类叶片微生物群（这些菌株的相应本地植物）时，在微米尺度上的空间组织。我们发现两种菌株的外来和常驻微生物细胞在几微米的距离处有显著的共定位。有趣的是，这种共定位部分是由于微生物细胞在新形成的荧光假单胞菌聚集物附近的优先附着。我们的结果表明，双向外来细菌-常驻微生物群相互作用影响叶片微生物群的微观空间组织，并且可能影响其他与表面相关的微生物群落。
