Esser Daniel S, Leveau Johan H J, Meyer Katrin M, Wiegand Kerstin
Department of Ecosystem Modelling, Büsgen-Institute, Georg-August-University of Göttingen, Büsgenweg 4, 37077 Göttingen, Germany
Department of Plant Pathology, University of California, Davis, CA 95616-8751, USA.
FEMS Microbiol Ecol. 2015 Mar;91(3). doi: 10.1093/femsec/fiu034. Epub 2014 Dec 24.
Microbial life on plant leaves is characterized by a multitude of interactions between leaf colonizers and their environment. While the existence of many of these interactions has been confirmed, their spatial scale or reach often remained unknown. In this study, we applied spatial point pattern analysis to 244 distribution patterns of Pantoea agglomerans and Pseudomonas syringae on bean leaves. The results showed that bacterial colonizers of leaves interact with their environment at different spatial scales. Interactions among bacteria were often confined to small spatial scales up to 5-20 μm, compared to interactions between bacteria and leaf surface structures such as trichomes which could be observed in excess of 100 μm. Spatial point-pattern analyses prove a comprehensive tool to determine the different spatial scales of bacterial interactions on plant leaves and will help microbiologists to better understand the interplay between these interactions.
植物叶片上的微生物生命以叶片定殖者与其环境之间的多种相互作用为特征。虽然其中许多相互作用的存在已得到证实，但其空间尺度或范围通常仍不为人知。在本研究中，我们对豆类叶片上成团泛菌和丁香假单胞菌的244个分布模式进行了空间点模式分析。结果表明，叶片上的细菌定殖者在不同的空间尺度上与其环境相互作用。与细菌和叶表面结构（如毛状体）之间的相互作用相比，细菌之间的相互作用通常局限于5-20μm的小空间尺度，而细菌与叶表面结构之间的相互作用可在超过100μm的尺度上观察到。空间点模式分析证明是确定植物叶片上细菌相互作用不同空间尺度的综合工具，将有助于微生物学家更好地理解这些相互作用之间的相互关系。
