Fu Fanyu, Huang Zeqing, Ye Hengli, Tan Biao, Wang Rongtian, Chen Weiheng
Guizhou University of Traditional Chinese Medicine, No. 50, Shidonglu, Nanming District, Guizhou, Guiyang 550002, China.
Department of Orthopaedics, Wangjing Hospital, China Academy of Chinese Medical Sciences, No. 6 Zhonghuannanlu, Chaoyang District, Beijing 100102, China.
Evid Based Complement Alternat Med. 2020 Sep 9;2020:7130105. doi: 10.1155/2020/7130105. eCollection 2020.
The Tao-Hong-Si-Wu-Tang (THSWT) formula, a classic prescription of traditional Chinese medicine, has long been used for the treatment of osteonecrosis of femoral head (ONFH). However, its mechanisms of action and molecular targets are not comprehensively clear. In the present study, the Traditional Chinese Medicine System Pharmacology (TCMSP) database was employed to retrieve the active compounds of each herb included in the THSWT formula. After identifying the drug targets of active compounds and disease targets of ONFH, intersection analysis was conducted to screen out the shared targets. The protein-protein network of the shared targets was built for further topological analysis. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis were then carried out. A gene pathway network was constructed to screen the core target genes. We identified 61 active compounds, 155 drug targets, and 5443 disease targets. However, intersection analysis only screened out 37 shared targets. Kaempferol, luteolin, and baicalein regulated the greatest number of targets associated with ONFH. The THSWT formula may regulate osteocyte function through specific biological processes, including responses to toxic substances and oxidative stress. The regulated pathways included the relaxin, focal adhesion, nuclear factor-B, toll-like receptor, and AGE/RAGE signaling pathways. , , and were the important target genes in the gene network associated with the THSWT formula for the treatment of ONFH. Therefore, the present study suggested that the THSWT formula has an action mechanism involving multiple compounds and network targets for the treatment of ONFH.
桃红四物汤（THSWT）是一种经典的中药方剂，长期以来一直用于治疗股骨头坏死（ONFH）。然而，其作用机制和分子靶点尚不完全清楚。在本研究中，利用中药系统药理学（TCMSP）数据库检索THSWT方剂中每种草药的活性成分。在确定活性成分的药物靶点和ONFH的疾病靶点后，进行交集分析以筛选出共同靶点。构建共同靶点的蛋白质-蛋白质网络进行进一步的拓扑分析。然后进行基因本体论和京都基因与基因组百科全书通路分析。构建基因通路网络以筛选核心靶基因。我们鉴定出61种活性成分、155个药物靶点和5443个疾病靶点。然而，交集分析仅筛选出37个共同靶点。山奈酚、木犀草素和黄芩素调节与ONFH相关的靶点数量最多。THSWT方剂可能通过特定的生物学过程调节骨细胞功能，包括对有毒物质和氧化应激的反应。所调节的通路包括松弛素、粘着斑、核因子-κB、Toll样受体和AGE/RAGE信号通路。 、 和 是与THSWT方剂治疗ONFH相关的基因网络中的重要靶基因。因此，本研究表明THSWT方剂治疗ONFH的作用机制涉及多种化合物和网络靶点。
