Department of Orthopedic Surgery, The Third Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Department of Oncology, The Third Affiliated Hospital of Anhui Medical University, 390 Huaihe Road, Hefei, Anhui, China.
Comput Math Methods Med. 2020 Sep 10;2020:1367576. doi: 10.1155/2020/1367576. eCollection 2020.
Many studies have shown that there are many circular RNA (circRNA) expression abnormalities in osteosarcoma (OS), and this abnormality is related to the development of osteosarcoma. But at present, it is unclear as to what circITGA7 has in the OS and what it does. In this study, qRT-PCR was used to detect the expression of circITGA7, miR-370, and PIM1 mRNA in OS tissues and cells. The CCK-8 assay was used to detect the effect of circITGA7 on cell proliferation. Later, the transwell assay was used to detect cell migration and invasion. The dual-luciferase reporter assay confirmed the existence of the targeting relationship between circITGA7 and miR-370, and miR-370 and PIM1. We found that circITGA7 was upregulated in OS tissues and cell lines. Knockdown of circITGA7 weakened the cell's ability to proliferate and metastasize. Furthermore, we observed that miR-370 was negatively regulated by circITGA7, while PIM1 was positively regulated by it. A functional assay validated that circITGA7 promoted OS progression via suppressing miR-370 and miR-370 affected OS proliferation and migration via PIM6 in OS. In summary, this study shows that circITGA7 promotes OS proliferation and metastasis via miR-370/PIM1.
许多研究表明，骨肉瘤（OS）中存在许多环状 RNA（circRNA）表达异常，这种异常与骨肉瘤的发生发展有关。但是目前，circITGA7 在骨肉瘤中起什么作用以及它的作用机制尚不清楚。在本研究中，我们通过 qRT-PCR 检测了骨肉瘤组织和细胞中 circITGA7、miR-370 和 PIM1 mRNA 的表达。CCK-8 检测表明 circITGA7 对细胞增殖有影响。之后，我们通过 Transwell 实验检测了细胞迁移和侵袭。双荧光素酶报告实验证实了 circITGA7 与 miR-370、miR-370 与 PIM1 之间存在靶向关系。我们发现 circITGA7 在骨肉瘤组织和细胞系中表达上调。circITGA7 的敲低削弱了细胞的增殖和转移能力。此外，我们观察到 circITGA7 负调控 miR-370，而 PIM1 则正调控 miR-370。功能实验验证了 circITGA7 通过抑制 miR-370 促进 OS 进展，而 miR-370 通过 PIM6 影响 OS 增殖和迁移。综上所述，本研究表明 circITGA7 通过 miR-370/PIM1 促进骨肉瘤的增殖和转移。
