Luo Gang, Li Guohao, Wan Zhihua, Zhang Yuanjie, Liu Dong, Guo Yonglian
Department of Urology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, China.
Department of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, China.
J Oncol. 2021 Dec 31;2021:8060389. doi: 10.1155/2021/8060389. eCollection 2021.
Prostate cancer (PCa) refers to one of the most common tumors in male's genitourinary system. Emerging research has confirmed that circRNAs play an important role in the occurrence and development of tumors. However, the correlation between circular RNA circITGA7 and PCa still remains unclear. Here, the role of circITGA7 in PCa was explored and the underlying mechanism was investigated as well. The circRNA expression profiles in PCa and the paracancerous tissues were established by high-throughput sequencing. The expression levels of circITGA7 in PCa tissues and cells were detected by qRT-PCR. Cell Counting Kit-8, colony formation, EdU, and flow cytometry assays were used to detect the effects of circITGA7 on PCa cell proliferation. To further explore the underlying mechanisms, bioinformatics analysis on downstream target genes was carried out. RNA immunoprecipitation and dual-luciferase reporter assays were used to verify the direct relationship between miR-370-3p and circITGA7 or P21. The present results demonstrated that circITGA7 was downregulated in PCa tissues and cells. Gain- or loss-of-function assays showed that circITGA7 inhibited the proliferation of PCa cells and . Mechanically, circITGA7 served as a sponge for miR-370-3p, and miR-370-3p could target P21 in PCa cells. The inhibition of cell proliferation induced by circITGA7 could be reversed by transfecting miR-370-3p mimic. Collectively, our data indicated that circITGA7 played an important role in inhibiting tumor proliferation in PCa and might be a potential therapeutic target.
前列腺癌（PCa）是男性泌尿生殖系统中最常见的肿瘤之一。新兴研究已证实环状RNA（circRNAs）在肿瘤的发生发展中起重要作用。然而，环状RNA circITGA7与前列腺癌之间的相关性仍不清楚。在此，我们探讨了circITGA7在前列腺癌中的作用，并研究了其潜在机制。通过高通量测序建立了前列腺癌组织和癌旁组织中的环状RNA表达谱。采用qRT-PCR检测前列腺癌组织和细胞中circITGA7的表达水平。使用细胞计数试剂盒-8、集落形成、EdU和流式细胞术检测circITGA7对前列腺癌细胞增殖的影响。为进一步探究潜在机制，对下游靶基因进行了生物信息学分析。采用RNA免疫沉淀和双荧光素酶报告基因检测验证miR-370-3p与circITGA7或P21之间的直接关系。目前的结果表明，circITGA7在前列腺癌组织和细胞中表达下调。功能获得或缺失实验表明，circITGA7抑制前列腺癌细胞的增殖。机制上，circITGA7作为miR-370-3p的海绵，miR-370-3p可靶向前列腺癌细胞中的P21。转染miR-370-3p模拟物可逆转circITGA7诱导的细胞增殖抑制。总的来说，我们的数据表明circITGA7在抑制前列腺癌肿瘤增殖中起重要作用，可能是一个潜在的治疗靶点。
