Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC, USA.
GOJO Industries Inc, Akron, OH, USA.
Lett Appl Microbiol. 2020 Dec;71(6):605-610. doi: 10.1111/lam.13393. Epub 2020 Oct 4.
Successful human norovirus (HuNoV) cultivation in stem cell-derived human intestinal enteroids (HIE) was recently reported. The purpose of this study was to evaluate the anti-HuNoV efficacy of two alcohol-based commercial hand sanitizers and 60% ethanol by suspension assay using RNase-RT-qPCR, with subsequent validation of efficacy by HuNoV cultivation using the HIE model. In suspension, when evaluated by RNase-RT-qPCR, 60% ethanol resulted in less than one log reduction in HuNoV genome equivalent copies (GEC) regardless of contact time (30 or 60s) or soil load. The two commercial products outperformed 60% ethanol regardless of contact time or soil load, providing 2·2-3·2 log HuNoV GEC reductions by suspension assay. Product B could not be validated in the HIE model due to cytotoxicity. Following a 60s exposure, viral replication in the HIE model increased 1·9 ± 0·2 log HuNoV GEC for the neutralization (positive) control and increased 0·9 ± 0·2 log HuNoV GEC in challenged HIE after treatment with 60% ethanol. No HuNoV replication in HIE was observed after a 60 s exposure to Product A.
最近有报道称，在干细胞衍生的人类肠道类器官（HIE）中成功培养了人类诺如病毒（HuNoV）。本研究的目的是通过使用 RNase-RT-qPCR 的悬浮试验评估两种含酒精的商业手部消毒剂和 60%乙醇对 HuNoV 的抗病毒功效，并随后使用 HIE 模型对其抗病毒功效进行验证。在悬浮状态下，通过 RNase-RT-qPCR 评估时，无论接触时间（30 秒或 60 秒）或土壤负荷如何，60%乙醇均导致 HuNoV 基因组等效拷贝数（GEC）减少不到 1 对数。两种商业产品的表现均优于 60%乙醇，无论接触时间或土壤负荷如何，均可通过悬浮试验减少 2.2-3.2 对数 HuNoV GEC。由于细胞毒性，产品 B 无法在 HIE 模型中进行验证。在 HIE 模型中，经过 60 秒的暴露后，中和（阳性）对照中 HuNoV 的复制增加了 1.9±0.2 对数 HuNoV GEC，而经 60%乙醇处理后，经挑战的 HIE 中 HuNoV 的复制增加了 0.9±0.2 对数 HuNoV GEC。在暴露于产品 A 60 秒后，未观察到 HIE 中 HuNoV 的复制。
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