Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
Laboratory of Virus Control, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
J Virol. 2024 Apr 16;98(4):e0166323. doi: 10.1128/jvi.01663-23. Epub 2024 Mar 12.
Human norovirus (HuNoV) is a major cause of acute gastroenteritis and foodborne diseases, affecting all age groups. Despite its clinical needs, no approved antiviral therapies are available. Since the discovery of HuNoV in 1972, studies on anti-norovirals, mechanism of HuNoV infection, viral inactivation, etc., have been hampered by the lack of a robust laboratory-based cultivation system for HuNoV. A recent breakthrough in the development of HuNoV cultivation systems has opened opportunities for researchers to investigate HuNoV biology in the context of HuNoV infections. A tissue stem cell-derived human intestinal organoid/enteroid (HIO) culture system is one of those that supports HuNoV replication reproducibly and, to our knowledge, is most widely distributed to laboratories worldwide to study HuNoV and develop therapeutic strategies. This review summarizes recently developed HuNoV cultivation systems, including HIO, and their use in antiviral studies.
人类诺如病毒（HuNoV）是急性胃肠炎和食源性疾病的主要病因，影响所有年龄段。尽管有临床需求，但目前尚无获得批准的抗病毒疗法。自 1972 年发现 HuNoV 以来，由于缺乏稳健的基于实验室的 HuNoV 培养系统，针对抗诺如病毒药物、HuNoV 感染机制、病毒失活等方面的研究受到了阻碍。最近，HuNoV 培养系统的开发取得了突破，为研究人员在 HuNoV 感染背景下研究 HuNoV 生物学提供了机会。一种组织干细胞衍生的人类肠道类器官（HIO）培养系统就是其中之一，它能够重复性地支持 HuNoV 复制，并且据我们所知，它在全球范围内分布最广，被用于研究 HuNoV 并开发治疗策略。本综述总结了最近开发的 HuNoV 培养系统，包括 HIO，及其在抗病毒研究中的应用。
