Virology Laboratory, Shengjing Hospital of China Medical University, Liaoning, China.
Eur Rev Med Pharmacol Sci. 2020 Sep;24(17):9196-9201. doi: 10.26355/eurrev_202009_22871.
The aim of this study is to find the distributions of pathogens in 164 suspected COVID-19 patients from the outpatient clinic of Shenjing Hospital of China Medical University from 24th January, 2020, to 29th February of 2020.
164 COVID-19 suspected patients were from the Shengjing Hospital of China Medical University. Oropharyngeal swab specimens were acquired by respiratory doctors under standardized conditions. Specific nucleic acids of SARS-CoV-2, influenza A and B, respiratory syncytial virus A and B, adenovirus, parainfluenza virus, along with pneumonic mycoplasma were detected by real-time fluorescence PCR. Symptomatic, epidemiologic, laboratory and radiological data of the patients were obtained from the electronic medical record system of our hospital.
Among the 164 patients, 3 were positive for SARS-CoV-2, 15 were positive for other respiratory viruses and 16 were positive for pneumonic mycoplasma. Of the positive patients above, 1 patient was co-infected with SARS-CoV-2 and adenovirus, and 1 was co-infected with influenza B and pneumonic mycoplasma. The 3 SARS-CoV-2 infected patients were clinically diagnosed as COVID-19 because they meet the diagnostic criteria listed in "Chinese Clinical Guidance for COVID-19 Pneumonia diagnosis and treatment", including epidemic history, symptom and pathogenic detection, as well as abnormalities of the laboratory and radiological data. However, the clinical characteristics of COVID-19 patients were non-specific compared to those of the patients infected with other respiratory viruses.
The endemic common respiratory pathogens are more prevalent than SARS-CoV-2 in the SARS-CoV-2 non-epidemic areas of this research. Detection of the pathogen is the unique means for definite COVID-19 diagnosis.
本研究旨在调查 2020 年 1 月 24 日至 2 月 29 日期间，中国医科大学盛京医院 164 例疑似 COVID-19 患者的病原体分布。
164 例 COVID-19 疑似患者来自中国医科大学盛京医院。呼吸科医生在标准化条件下采集咽拭子标本。采用实时荧光 PCR 检测 SARS-CoV-2、甲型和乙型流感病毒、呼吸道合胞病毒 A 和 B、腺病毒、副流感病毒以及肺炎支原体的特异性核酸。从我院电子病历系统获取患者的症状、流行病学、实验室和影像学数据。
在 164 例患者中，3 例 SARS-CoV-2 阳性，15 例其他呼吸道病毒阳性，16 例肺炎支原体阳性。上述阳性患者中，1 例合并 SARS-CoV-2 和腺病毒感染，1 例合并乙型流感病毒和肺炎支原体感染。3 例 SARS-CoV-2 感染患者因符合“中国 COVID-19 肺炎诊断与治疗临床指南”中列出的诊断标准，包括流行病学史、症状和病原学检测，以及实验室和影像学数据异常，被临床诊断为 COVID-19。然而，与感染其他呼吸道病毒的患者相比，COVID-19 患者的临床特征不具有特异性。
在本研究 SARS-CoV-2 非流行地区，地方性常见呼吸道病原体比 SARS-CoV-2 更为流行。病原体检测是明确 COVID-19 诊断的唯一手段。
