辐照藜麦（Chenopodium quinoa）的植物化学成分和抗氧化活性评价。

Evaluation of phytochemicals and antioxidant activity of gamma irradiated quinoa (Chenopodium quinoa).

机构信息

Atomic Energy Authority, Nuclear Research Center, Plant Research Department, Cairo, Egypt.

出版信息

Braz J Biol. 2021 Jul-Sep;81(3):806-813. doi: 10.1590/1519-6984.232270. Epub 2021 Aug 31.

Abstract

Phytochemical and antioxidant activity of quinoa flour was evaluated after subjected to gamma irradiation processes at dose 3 and 6 kGy. Both non-irradiated and irradiated quinoa samples were subjected to successive extractions in ethanol solvent. The antioxidant activity after gamma irradiation treatment was investigated via Ferric reducing antioxidant power (FRAP) and radical-scavenging activity (RSA) using 2,2-diphenyl-1-picrylhydrazyl (DPPH).Total phenolic and flavonoid content were analyzed using Folin-Ciocalteu micro-method, aluminium chloride (AlCl3) method and High Performance Liquid Chromatography (HPLC). As well as, effect of irradiation treatment on saponin was also evaluated. Irradiation treatment showed slight differences in the saponin content after exposure to 3 and 6 kGy. Irradiation process enhanced both total phenolic content (TPC) and Total flavonoid content (TFC), TPC were 34.52 and 30.92 mg Gallic Acid Equivalent (GAE)/100g compared to 26.25 mg GAE/100g in non-irradiated quinoa. TFC were 67.44 and 62.89 mg Quercetin Equivalents (QE)/100g compared to 53.15 mg QE/100g. Irradiation dose 3 kGy significantly (p> 0.05) decreased the IC50 as DPPH-RSA and increased the FRAP.

摘要

藜麦粉的植物化学物质和抗氧化活性在经过 3 和 6 kGy 的伽马辐射处理后进行了评估。未经辐射和辐射处理的藜麦样品均在乙醇溶剂中进行连续提取。通过 2,2-二苯基-1-苦基肼基（DPPH）自由基清除活性（RSA）和铁还原抗氧化能力（FRAP）来研究伽马辐射处理后的抗氧化活性。使用福林-肖卡尔特微法、三氯化铝（AlCl3）法和高效液相色谱法（HPLC）分析总酚和类黄酮含量。以及，还评估了辐照处理对皂苷的影响。辐照处理后，在暴露于 3 和 6 kGy 时，皂苷含量略有差异。辐照过程增强了总酚含量（TPC）和总类黄酮含量（TFC），TPC 分别为 34.52 和 30.92 mg 没食子酸当量（GAE）/100g，而未辐照藜麦为 26.25 mg GAE/100g。TFC 分别为 67.44 和 62.89 mg 槲皮素当量（QE）/100g，而未辐照藜麦为 53.15 mg QE/100g。3 kGy 的辐照剂量显著（p>0.05）降低了 DPPH-RSA 的 IC50，并增加了 FRAP。