Rautelin H
Acta Pathol Microbiol Immunol Scand B. 1987 Apr;95(2):103-8. doi: 10.1111/j.1699-0463.1987.tb03095.x.
Acid and alkaline extracts from several Campylobacter strains were used as antigens in enzyme immunoassay (EIA). Antibodies were detected in rabbits immunized with formalin-treated, boiled, or autoclaved whole bacteria of different Campylobacter subspecies. For the preparation of the extracts, three Campylobacter fetus subsp. venerealis strains, three Campylobacter fetus subsp. fetus strains, four Campylobacter coli, and five Campylobacter jejuni strains were used. The acid extracts of Campylobacter fetus subsp. fetus and Campylobacter fetus subsp. venerealis were antigenically similar to each other but different from Campylobacter coli and Campylobacter jejuni strains. These acid extracts were found to be suitable antigens in enzyme immunoassay. However, when alkaline extracts were used as the antigen, fewer antisera reacted with them than with the acid extracts, and many pre-immune sera gave high values.
几种弯曲杆菌菌株的酸性和碱性提取物被用作酶免疫测定（EIA）中的抗原。在用不同弯曲杆菌亚种的经福尔马林处理、煮沸或高压灭菌的全菌免疫的兔子中检测到了抗体。为了制备提取物，使用了三株胎儿弯曲杆菌性病亚种菌株、三株胎儿弯曲杆菌胎儿亚种菌株、四株空肠弯曲菌和五株结肠弯曲菌菌株。胎儿弯曲杆菌胎儿亚种和胎儿弯曲杆菌性病亚种的酸性提取物在抗原性上彼此相似，但与结肠弯曲菌和空肠弯曲菌菌株不同。这些酸性提取物被发现是酶免疫测定中合适的抗原。然而，当使用碱性提取物作为抗原时，与它们反应的抗血清比与酸性提取物反应的抗血清少，并且许多免疫前血清的值很高。
