Kosunen T U, Danielsson D, Kjellander J
Acta Pathol Microbiol Immunol Scand B. 1982 Jun;90(3):191-6.
Antisera against 12 campylobacter strains (8 C. fetus ss. jejuni, 2 ss fetus and 2 ss. intestinalis) were obtained from rabbits immunized with formalin treated bacteria. Cross testing of live boiled and autoclaved organisms of these strains were performed with direct slide and tube agglutinations, and with agglutinations of latex particles and protein A-containing staphylococci coated with antibodies. Confirmed by absorption experiments both heat labile and heat stable antigens were demonstrated. The highest specificity for serological differentiation was obtained with slide and latex agglutination with live bacteria, which were subsequently used for differentiation of C. fetus ss. jejuni strains. For this purpose we applied 22 C. fetus ss. jejuni antisera absorbed with boiled homologous bacteria. Clearcut homologous reactions, with few and weak crossreactions, indicated the presence of a variety of heat labile antigens, which could form a basis for serotyping. Preliminary typing of 170 clinical isolates with slide agglutination using 17 unabsorbed C. fetus ss. jejuni antisera demonstrated the applicability of this approach for epidemiological studies. We found antigenically similar strains in family outbreaks, antigenic differences in strains of different origin, new combinations of cross reactions not seen in strains used in immunization, antigenically "new" strains not reacting with these antisera, and the dominance of a few antigenic types among the isolates.
从用甲醛处理过的细菌免疫的兔子身上获得了针对12种弯曲杆菌菌株（8株空肠弯曲菌胎儿亚种、2株胎儿弯曲菌亚种和2株肠道弯曲菌亚种）的抗血清。对这些菌株的活菌、煮沸菌和高压灭菌菌进行了交叉检测，采用直接玻片凝集试验、试管凝集试验、乳胶颗粒凝集试验以及包被有抗体的含A蛋白葡萄球菌凝集试验。通过吸收试验证实了热不稳定抗原和热稳定抗原的存在。用活细菌进行玻片凝集试验和乳胶凝集试验对血清学鉴别具有最高的特异性，随后用于空肠弯曲菌胎儿亚种菌株的鉴别。为此，我们应用了22种用煮沸的同源细菌吸收过的空肠弯曲菌胎儿亚种抗血清。清晰的同源反应，交叉反应少且弱，表明存在多种热不稳定抗原，这可为血清分型提供基础。用17种未吸收的空肠弯曲菌胎儿亚种抗血清通过玻片凝集试验对170株临床分离株进行初步分型，证明了该方法在流行病学研究中的适用性。我们在家庭暴发中发现了抗原相似的菌株，在不同来源的菌株中发现了抗原差异，在免疫所用菌株中未见的交叉反应新组合，不与这些抗血清反应的抗原性“新”菌株，以及分离株中少数抗原类型占主导地位。
