Rautelin H, Kosunen T U
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Acta Pathol Microbiol Immunol Scand B. 1987 Oct;95(5):277-81. doi: 10.1111/j.1699-0463.1987.tb03125.x.
Campylobacter IgA, IgM and IgG antibody titers of 442 patients with positive isolations of Campylobacter were tested in enzyme immunoassay (EIA). Of the patients, 330 showed elevated antibody end-point titers to a single acid extract antigen prepared from a C. jejuni strain. Sixteen further patients had fourfold or greater antibody titer changes in paired sera. Nine patients, who did not show elevated antibody titers for the single extract, had positive serological responses for a multiple acid extract prepared from six C. jejuni/coli and a C. fetus subsp. fetus strain. The specificity of the test, based on Campylobacter antibodies of 200 healthy blood donors, was 99%. The use of the single acid extract gave a sensitivity of 78%. The additional use of the multiple acid extract raised the sensitivity to 80%.
采用酶免疫测定法（EIA）检测了442例弯曲杆菌分离阳性患者的弯曲杆菌IgA、IgM和IgG抗体滴度。其中330例患者对从空肠弯曲菌菌株制备的单一酸提取物抗原有升高的抗体终点滴度。另有16例患者配对血清中的抗体滴度有四倍或更高的变化。9例对单一提取物抗体滴度未升高的患者，对由6株空肠弯曲菌/大肠弯曲菌和1株胎儿弯曲菌胎儿亚种制备的多种酸提取物有阳性血清学反应。基于200名健康献血者的弯曲杆菌抗体，该检测的特异性为99%。使用单一酸提取物时灵敏度为78%。额外使用多种酸提取物可将灵敏度提高到80%。
