Rautelin H I, Kosunen T U
Department of Bacteriology and Immunology, University of Helsinki, Finland.
J Clin Microbiol. 1987 Oct;25(10):1944-51. doi: 10.1128/jcm.25.10.1944-1951.1987.
Campylobacter antibodies of the immunoglobulin G (IgG), IgM, and IgA classes were determined by enzyme immunoassay with acid glycine extract antigen in patients and controls involved in two Campylobacter outbreaks and in 266 unselected patients with acute enteritis. The assay showed a specificity of 99% for each immunoglobulin class in sera from 200 healthy blood donors. Elevated Campylobacter antibody titers were shown in 97% of stool culture-positive patients involved in the outbreaks. Rapid changes of IgA and IgM Campylobacter antibodies were typical of the early phase of serologic response in the outbreaks and thus offered the best diagnostic value in the serologic diagnosis of acute campylobacteriosis. In unselected patients with acute enteritis, the assay revealed elevated Campylobacter antibody titers in 37 patients, of whom only 12 had had positive Campylobacter stool cultures. In the sera of patients with other bacterial findings in addition to high titers of Campylobacter antibodies, no cross-reacting antibodies were found, but there was evidence of several mixed infections.
采用酶免疫分析法，以酸性甘氨酸提取物抗原检测了两起弯曲杆菌暴发疫情中的患者及对照，以及266例未经挑选的急性肠炎患者血清中免疫球蛋白G（IgG）、IgM和IgA类别的弯曲杆菌抗体。该检测方法对200名健康献血者血清中每种免疫球蛋白类别的特异性为99%。在暴发疫情中，97%粪便培养呈阳性的患者弯曲杆菌抗体滴度升高。在疫情中，IgA和IgM弯曲杆菌抗体的快速变化是血清学反应早期阶段的典型特征，因此在急性弯曲杆菌病的血清学诊断中具有最佳诊断价值。在未经挑选的急性肠炎患者中，该检测方法显示37例患者弯曲杆菌抗体滴度升高，其中只有12例弯曲杆菌粪便培养呈阳性。在除高滴度弯曲杆菌抗体外还有其他细菌检测结果的患者血清中，未发现交叉反应抗体，但有证据表明存在几种混合感染。
