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复制人类基因组中姐妹染色单体的构象。

Conformation of sister chromatids in the replicated human genome.

机构信息

Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna BioCenter, Vienna, Austria.

Institute of Organic Chemistry and Center for Molecular Biosciences (CMBI), Leopold-Franzens University, Innsbruck, Austria.

出版信息

Nature. 2020 Oct;586(7827):139-144. doi: 10.1038/s41586-020-2744-4. Epub 2020 Sep 23.

DOI:10.1038/s41586-020-2744-4
PMID:32968280
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7116725/
Abstract

The three-dimensional organization of the genome supports regulated gene expression, recombination, DNA repair, and chromosome segregation during mitosis. Chromosome conformation capture (Hi-C) analysis has revealed a complex genomic landscape of internal chromosomal structures in vertebrate cells, but the identical sequence of sister chromatids has made it difficult to determine how they topologically interact in replicated chromosomes. Here we describe sister-chromatid-sensitive Hi-C (scsHi-C), which is based on labelling of nascent DNA with 4-thio-thymidine and nucleoside conversion chemistry. Genome-wide conformation maps of human chromosomes reveal that sister-chromatid pairs interact most frequently at the boundaries of topologically associating domains (TADs). Continuous loading of a dynamic cohesin pool separates sister-chromatid pairs inside TADs and is required to focus sister-chromatid contacts at TAD boundaries. We identified a subset of TADs that are overall highly paired and are characterized by facultative heterochromatin and insulated topological domains that form separately within individual sister chromatids. The rich pattern of sister-chromatid topologies and our scsHi-C technology will make it possible to investigate how physical interactions between identical DNA molecules contribute to DNA repair, gene expression, chromosome segregation, and potentially other biological processes.

摘要

基因组的三维组织支持有丝分裂过程中基因表达的调控、重组、DNA 修复和染色体分离。染色体构象捕获（Hi-C）分析揭示了脊椎动物细胞中内部染色体结构的复杂基因组景观，但由于姐妹染色单体的相同序列，很难确定它们在复制染色体中如何拓扑相互作用。在这里，我们描述了基于 4-硫代胸腺嘧啶和核苷转换化学标记新生 DNA 的姐妹染色单体敏感 Hi-C（scsHi-C）。人类染色体的全基因组构象图谱显示，姐妹染色单体对在拓扑关联域（TAD）的边界处最频繁地相互作用。动态黏连蛋白池的连续加载将 TAD 内的姐妹染色单体对分开，并且需要将姐妹染色单体在 TAD 边界处的接触集中起来。我们鉴定了一组总体上高度配对的 TAD，其特征是组成性异染色质和独立形成于单个姐妹染色单体内部的隔离拓扑域。姐妹染色单体拓扑的丰富模式和我们的 scsHi-C 技术将使研究相同 DNA 分子之间的物理相互作用如何有助于 DNA 修复、基因表达、染色体分离以及潜在的其他生物学过程成为可能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcf9/7116725/8d3c8d758702/EMS114563-f014.jpg
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2
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Nat Methods. 2020 Mar;17(3):261-272. doi: 10.1038/s41592-019-0686-2. Epub 2020 Feb 3.
3
Thioguanosine Conversion Enables mRNA-Lifetime Evaluation by RNA Sequencing Using Double Metabolic Labeling (TUC-seq DUAL).
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Nat Struct Mol Biol. 2025 Jul 4. doi: 10.1038/s41594-025-01604-7.
4
Chemotherapy-Mediated Induction of PD-L1 via SEI1 Facilitates Myeloma Immune Evasion.化疗通过SEI1介导的PD-L1诱导促进骨髓瘤免疫逃逸。
Adv Sci (Weinh). 2025 May;12(19):e2411082. doi: 10.1002/advs.202411082. Epub 2025 Mar 26.
5
Extensive mutual influences of SMC complexes shape 3D genome folding.SMC复合物的广泛相互影响塑造了三维基因组折叠。
Nature. 2025 Apr;640(8058):543-553. doi: 10.1038/s41586-025-08638-3. Epub 2025 Feb 26.
6
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