半合成苯丙氨酸B1-氚标记胰岛素的改进制备方法。
Improved preparation of semisynthetic PheB1-tritiated insulin.
作者信息
Grant K I, von Holt C
出版信息
Biol Chem Hoppe Seyler. 1987 Mar;368(3):239-48. doi: 10.1515/bchm3.1987.368.1.239.
PMID:3297097
Abstract
Semisynthetic [PheB1-3H]insulin was prepared. Each intermediate in the synthesis was characterised by HPLC, polyacrylamide gel electrophoresis and end group determination by dansylation. The end product had a specific activity of up to 59 Ci/mmol. Semisynthetic insulin proved indistinguishable from natural insulin in its chemical and immunological properties and was shown to be biologically fully active by in vivo and in vitro assays.
摘要
制备了半合成的[苯丙氨酸B1 - 3H]胰岛素。合成过程中的每个中间体都通过高效液相色谱法（HPLC）、聚丙烯酰胺凝胶电泳以及丹磺酰化法进行端基测定来表征。最终产物的比活度高达59居里/毫摩尔。半合成胰岛素在化学和免疫特性上与天然胰岛素无异，并且通过体内和体外试验表明具有完全的生物活性。
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