J Biomed Nanotechnol. 2020 Apr 1;16(4):505-519. doi: 10.1166/jbn.2020.2915.
The clinical studies proved the adverse effect of Propranolol on sexual function. Regarding this issue, the key research question of this study was, whether the designed herbal nanohybrid formula EGCG-chitosan-alginate has an efficacy versus Propranolol, or not? The formula was optimized according to the coacervation method. Its molecular structure and characteristics were confirmed. The entrapment efficiency was determined, and the stability, as well release study was conducted. The study was conducted for 65 days. To answer the raised question, tissue weights of the testis, epididymis seminal vesicles, and prostate were determined. Oxidative stress markers as MDA and GSH were measured in testis and epididymis, while testosterone in blood serum. The semen analysis was performed. DNA damage was detected according to the comet assay procedures. Conventional pathological examination was done in special concern to testis and epididymis. The characterization results reflected the good preparation of the formula with an amorphous structure in a range of 200 nm, high stability with ZP + 57.3 mV. The calculated EE was 84.10 ± 1.19% and the release percent was 72.11 ± 0.77% for 24 hrs. All the rats increased in the weight with variations among the groups in the tissue organs. The finding exposed a significant decrease in the average of MDA in the rats' testes with a significant increase in GSH while a non-significant difference in the epididymis, in both. The testosterone, the seminal parameters, and the DNA integrity significantly increased in the nano-formula compared to propranolol. Likewise, normal pathological findings of the nano-formula in the testis and Epididymis compared to abnormal of propranolol. In total, the current research confirmed that EGCG had no toxic effects and able to promote fertility. The most important finding was the administration of EGCG either in normal or nano-form prior to propranolol alleviated the effects of propranolol. These findings reflected the protection evident of EGCG versus propranolol.
临床研究证实了普萘洛尔对性功能的不良影响。关于这个问题，本研究的关键研究问题是，设计的表没食子儿茶素没食子酸酯-壳聚糖-海藻酸钠草药纳米杂交配方是否比普萘洛尔更有效？该配方是根据共凝聚法进行优化的。其分子结构和特性得到了确认。测定了包封效率，并进行了稳定性和释放研究。研究进行了 65 天。为了回答提出的问题，测定了睾丸、附睾、精囊和前列腺的组织重量。在睾丸和附睾中测量了氧化应激标志物 MDA 和 GSH，在血清中测量了睾酮。进行了精液分析。根据彗星分析程序检测 DNA 损伤。特别关注睾丸和附睾进行常规病理检查。特征化结果反映了配方的良好制备，其在 200nm 范围内具有无定形结构，具有+57.3mV 的高稳定性。计算的 EE 为 84.10±1.19%，24 小时内的释放百分比为 72.11±0.77%。所有大鼠的体重都增加了，组织器官的各组之间存在差异。结果表明，大鼠睾丸中 MDA 的平均水平显著下降，GSH 显著增加，而附睾中则无显著差异。与普萘洛尔相比，纳米配方中的睾酮、精液参数和 DNA 完整性显著增加。同样，纳米配方在睾丸和附睾中的正常病理发现与普萘洛尔的异常相比。总的来说，目前的研究证实，表没食子儿茶素没食子酸酯没有毒性作用，能够促进生育能力。最重要的发现是，在给予普萘洛尔之前，无论是以正常形式还是纳米形式给予表没食子儿茶素没食子酸酯，都能减轻普萘洛尔的作用。这些发现反映了表没食子儿茶素没食子酸酯对普萘洛尔的保护作用。
