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利用……生物合成氧化锌纳米颗粒:表征及其对促进……植物生长的营养意义

Biosynthesis of ZnO Nanoparticles Using : Characterization and Nutritive Significance for Promoting Plant Growth in L.

作者信息

Sabir Sumera, Zahoor Muhammad Asif, Waseem Muhammad, Siddique Muhammad Hussnain, Shafique Muhammad, Imran Muhammad, Hayat Sumreen, Malik Imran Riaz, Muzammil Saima

机构信息

Department of Microbiology, Government College University, Faisalabad, Pakistan.

Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan.

出版信息

Dose Response. 2020 Sep 14;18(3):1559325820958911. doi: 10.1177/1559325820958911. eCollection 2020 Jul-Sep.

Abstract

Nano-fertilizer(s), an emerging field of agriculture, is alternate option for enhancement of plant growth replacing the synthetic fertilizers. Zinc oxide nanoparticles (ZnO NPs) can be used as the zinc source for plants. The present investigation was carried out to assess the role of ZnO NPs in growth promotion of maize plants. Biosynthesized ZnO NPs (using sp) were characterized using Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), X-ray diffraction (XRD) and Zeta potential. Different concentrations of ZnO NPs (2, 4, 8, 16 mg/L) were explored in pot culture experiment. Size of ZnO NPs ranged between 16 and 20 nm. A significant increase in growth parameters like shoot length (61.7%), root length (56.9%) and significantly higher level of protein was observed in the treated plants. The overall pattern for growth biomarkers including the protein contents was maximum at 8 mg/L of ZnO NPs. It was observed that application of biosynthesized ZnO NPs has improved majority of growth biomarkers including plant growth parameters, protein contents and leaf area. Therefore, biosynthesized ZnO NPs could be considered as an alternate source of nutrient in Zn deficient soils for promoting the modern agriculture.

摘要

纳米肥料作为农业领域一个新兴的方向,是替代合成肥料以促进植物生长的选择。氧化锌纳米颗粒(ZnO NPs)可作为植物的锌源。本研究旨在评估ZnO NPs在促进玉米植株生长方面的作用。利用扫描电子显微镜(SEM)、透射电子显微镜(TEM)、X射线衍射(XRD)和zeta电位对生物合成的ZnO NPs(使用 sp)进行了表征。在盆栽试验中探究了不同浓度的ZnO NPs(2、4、8、16 mg/L)。ZnO NPs的尺寸在16至20纳米之间。在处理过的植株中,观察到生长参数如茎长(增加61.7%)、根长(增加56.9%)有显著增加,且蛋白质水平显著更高。包括蛋白质含量在内的生长生物标志物的总体模式在ZnO NPs浓度为8 mg/L时最高。据观察,生物合成的ZnO NPs的应用改善了大多数生长生物标志物,包括植物生长参数、蛋白质含量和叶面积。因此,生物合成的ZnO NPs可被视为缺锌土壤中促进现代农业的一种替代养分来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/7493260/3ccc800e340f/10.1177_1559325820958911-fig1.jpg

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