Wang Jun-Jie, Niu Meng-Han, Zhang Teng, Shen Wei, Cao Hong-Guo
Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao, China.
Front Physiol. 2020 Aug 18;11:1019. doi: 10.3389/fphys.2020.01019. eCollection 2020.
Long non-coding RNA (lncRNA) is involved in many biological processes, and it has been closely investigated. However, research into the role of lncRNA in ovine ovarian development is scant and poorly understood, particularly in relation to the molecular mechanisms of ovine oocyte maturation. In the current study, RNA sequencing was performed with germinal vesicle (GV) and in vitro matured metaphase II (MII) stage oocytes, isolated from ewes. Through the use of bioinformatic analysis, abundant candidate lncRNAs in stage-specific ovine oocytes were identified, and their - and -regulatory effects were deeply dissected using computational prediction software. Functional enrichment analysis of these lncRNAs revealed that they were involved in the regulation of many key signaling pathways during ovine oocyte development, which was reflected by their targeted genes. From this study, multiple lncRNA-mRNA networks were presumed to be involved in key signaling pathways regarding ovine oocyte maturation and meiotic resumption. In particular, one novel lncRNA (MSTRG.17927) appeared to mediate the regulation of phosphatidylinositol 3-kinase signaling (PI3K) signaling during ovine oocyte maturation. Therefore, this research offers novel insights into the molecular mechanisms underlying ovine oocyte meiotic maturation regulated by lncRNA-mRNA networks from a genome-wide perspective.
长链非编码RNA（lncRNA）参与许多生物学过程，并且已经得到了深入研究。然而，关于lncRNA在绵羊卵巢发育中的作用的研究却很少，人们对此了解甚少，特别是在绵羊卵母细胞成熟的分子机制方面。在本研究中，对从母羊分离出的生发泡（GV）期和体外成熟的中期II（MII）期卵母细胞进行了RNA测序。通过生物信息学分析，鉴定出了特定阶段绵羊卵母细胞中丰富的候选lncRNA，并使用计算预测软件深入剖析了它们的调控作用。对这些lncRNA的功能富集分析表明，它们参与了绵羊卵母细胞发育过程中许多关键信号通路的调控，这在它们的靶基因中得到了体现。从这项研究中，推测多个lncRNA- mRNA网络参与了与绵羊卵母细胞成熟和减数分裂恢复相关的关键信号通路。特别是，一种新型lncRNA（MSTRG.17927）似乎在绵羊卵母细胞成熟过程中介导了磷脂酰肌醇3-激酶信号通路（PI3K）的调控。因此，本研究从全基因组角度为lncRNA- mRNA网络调控绵羊卵母细胞减数分裂成熟的分子机制提供了新的见解。
