Zhang Tianyu, Zhang Ying, Ji Andong, Shi Runjia, Li Huiying, Zeng Qiangcheng
College of Life Sciences Dezhou University Dezhou Shandong China.
Institute of Nutrition and Health Qingdao University Qingdao China.
Food Sci Nutr. 2025 Feb 27;13(3):e70000. doi: 10.1002/fsn3.70000. eCollection 2025 Mar.
Essential fatty acids could regulate inflammation, especially n-3 PUFA (n-3 polyunsaturated fatty acids), which are considered to have a protective effect to inhibit neuroinflammation. Peony seed oil is one of the most abundant n-3 PUFAs in oils. but the mechanism of peony seed oil affecting inflammation in mice brains is still lacking convincing evidence. Sixty male C57BL/6J mice were randomly allocated into four groups: D-gal (D-galactose) induced model group, FO (D-gal + fish oil), PSO (D-gal + peony seed oil). After 10 weeks, the fatty acid composition in liver and brain tissues and potentially related genes were examined. Docosahexaenoic acid (DHA) was significantly higher, while arachidonic acid (AA) was significantly lower in both in the PSO and FO groups than that in the model group in the brain and liver. In the PSO and FO groups, the relative mRNA levels of , , and were significantly up-regulated, but and were significantly down-regulated compared to the model group. In the PSO and FO groups, the relative protein levels of PPARG, RXRA, and IL-10 were significantly up-regulated, and the expressions of AGERs, TNF-α, PLA2, and PGF2α were significantly down-regulated compared to the model group. The phosphorylation-tau of total tau protein ratio was significantly lower in the PSO and FO groups than in the model group. Peony seed oil, rich in n-3 PUFA, inhibited neuroinflammation and rescued the disruption of alternative splicing of the gene by activating the PPAR/RXR signaling pathway and promoting n-3/n-6 biosynthesis.
必需脂肪酸可以调节炎症，尤其是n-3多不饱和脂肪酸（n-3 PUFA），其被认为具有抑制神经炎症的保护作用。牡丹籽油是含n-3多不饱和脂肪酸最丰富的油脂之一。但牡丹籽油影响小鼠脑内炎症的机制仍缺乏令人信服的证据。将60只雄性C57BL/6J小鼠随机分为四组：D-半乳糖（D-galactose）诱导模型组、鱼油组（D-gal+鱼油）、牡丹籽油组（D-gal+牡丹籽油）。10周后，检测肝脏和脑组织中的脂肪酸组成以及潜在相关基因。在脑组织和肝脏中，牡丹籽油组和鱼油组的二十二碳六烯酸（DHA）显著升高，而花生四烯酸（AA）显著低于模型组。与模型组相比，牡丹籽油组和鱼油组中 、 和 的相对mRNA水平显著上调，但 和 显著下调。与模型组相比，牡丹籽油组和鱼油组中PPARG、RXRA和IL-10的相对蛋白水平显著上调，而AGERs、TNF-α、PLA2和PGF2α的表达显著下调。牡丹籽油组和鱼油组中总tau蛋白的磷酸化-tau比率显著低于模型组。富含n-3多不饱和脂肪酸的牡丹籽油通过激活PPAR/RXR信号通路和促进n-3/n-6生物合成来抑制神经炎症，并挽救 基因可变剪接的破坏。
