College of Life Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Gene. 2021 Jul 30;791:145716. doi: 10.1016/j.gene.2021.145716. Epub 2021 May 11.
Long non-coding RNA (lncRNA), a type of non-protein coding transcripts with lengths exceeding 200 nucleotides, is reported to be widely involved in many cellular and developmental processes. However, few roles of lncRNA in oocyte development have been defined. In this study, to uncover the effect of lncRNA during oocyte maturation, bovine germinal vesicle (GV) and in vitro matured metaphase II (MII) oocytes underwent RNA sequencing. Results revealed a wealth of candidate lncRNAs, which might participate in the biological processes of stage-specific oocytes. Furthermore, their trans- and cis-regulatory effects were investigated in-depth by using bioinformatic software. Functional enrichment analysis of target genes showed that these lncRNAs were likely involved in the regulation of many key signaling pathways during bovine oocyte maturation from GV to MII stage, as well as multiple lncRNA-mRNA networks. One novel lncRNA (MSTRG.19140) was particularly interesting, as it appeared to mediate the regulation of oocyte meiotic resumption, progesterone-mediated oocyte maturation, and cell cycle. Therefore, this study enhanced insights into the regulation of molecular mechanisms of bovine oocyte maturation from a lncRNA-mRNA network perspective.
长非编码 RNA（lncRNA）是一种长度超过 200 个核苷酸的非蛋白编码转录本，据报道广泛参与许多细胞和发育过程。然而，lncRNA 在卵母细胞发育中的作用尚未确定。在这项研究中，为了揭示 lncRNA 在卵母细胞成熟过程中的作用，牛生发泡（GV）和体外成熟的中期 II（MII）卵母细胞进行了 RNA 测序。结果揭示了大量候选 lncRNA，它们可能参与了阶段特异性卵母细胞的生物学过程。此外，还通过使用生物信息学软件深入研究了它们的顺式和反式调节作用。靶基因的功能富集分析表明，这些 lncRNA 可能参与了牛卵母细胞从 GV 到 MII 阶段成熟过程中许多关键信号通路的调节，以及多个 lncRNA-mRNA 网络。一个新的 lncRNA（MSTRG.19140）特别有趣，因为它似乎介导了卵母细胞减数分裂恢复、孕酮介导的卵母细胞成熟和细胞周期的调节。因此，这项研究从 lncRNA-mRNA 网络的角度增强了对牛卵母细胞成熟分子机制调节的认识。
