Sanitary-Epidemiological Station, Prądnicka 76, 31-202, Kraków, Poland.
The Chair and Department of Oral Surgery, Medical University of Lublin, Chodźki 6, 20-093, Lublin, Poland.
Lasers Med Sci. 2021 Jun;36(4):875-887. doi: 10.1007/s10103-020-03151-7. Epub 2020 Sep 26.
The aim of the study was to find variant of diode laser (λ = 810 nm) irradiation, which ensures elimination of unwanted microorganisms, including Rothia aeria, from dental healing abutments, and consequently accelerates process of wound healing in implantologically treated patients. The scope of the study included identification of the most contaminated areas on healing abutments, identification of microorganisms inhabiting various environments of oral cavities, assessment of effectiveness of various laser decontamination parameters against detected microorganisms (preliminary studies) and assessment of wound healing in patients after applying abutments with low roughness and optimal variant of laser irradiation (clinical studies). Imaging of surfaces of the healing abutments was performed using vertical scanning interferometry, scanning electron microscopy and optical microscopy. Microorganisms inhabiting the healing abutments, teeth and saliva from tested patients were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Three programmes of near-infrared diode laser at average powers of 1.00-3.84 W with two variants of exposure time were used for optimisation of laser parameters. Observation of wound healing was performed for 100 patients during 20 days after installation of abutments. On surfaces of the used healing abutments, a large number of microorganisms, with a predominance of R. aeria, were found. Irradiation with periimplantitis surgical programme for 30 s resulted in 99-100% reduction in the number of R. aeria and other microorganisms, depending on type of abutment (in vivo). The use of diode laser in the selected variant accelerates wound healing and provides complete elimination of pathogenic R. aeria and other microorganisms inhabiting surfaces of the healing abutments without marks.
本研究旨在寻找一种二极管激光（λ=810nm）照射的变体，以确保消除包括罗瑟氏菌（Rothia aeria）在内的不需要的微生物，从而加速植入物治疗患者的伤口愈合过程。本研究的范围包括确定愈合基台上污染最严重的区域，鉴定各种口腔环境中存在的微生物，评估各种激光消毒参数对检测到的微生物的有效性（初步研究），以及评估应用低粗糙度和最佳激光照射变体的基台后患者的伤口愈合情况（临床研究）。使用垂直扫描干涉测量法、扫描电子显微镜和光学显微镜对愈合基台的表面进行成像。使用基质辅助激光解吸/电离飞行时间质谱法鉴定来自测试患者的愈合基台、牙齿和唾液中栖息的微生物。使用平均功率为 1.00-3.84W 的三种近红外二极管激光器，采用两种暴露时间变体，对激光参数进行优化。在安装基台后的 20 天内，对 100 名患者的伤口愈合情况进行观察。在使用的愈合基台上的表面，发现了大量的微生物，其中罗瑟氏菌（Rothia aeria）占优势。针对种植体周围炎的手术方案进行 30 秒的照射，可使 R. aeria 和其他微生物的数量减少 99-100%，具体取决于基台类型（体内）。选择的变体中使用二极管激光可加速伤口愈合，并能完全消除栖息在愈合基台表面的致病性罗瑟氏菌（Rothia aeria）和其他微生物，而不会留下痕迹。
