Wawrzyk Anna, Łobacz Michał, Adamczuk Agnieszka, Sofińska-Chmiel Weronika, Wilczyński Sławomir, Rahnama Mansur
Silesian Park of Medical Technology Kardio-Med Silesia in Zabrze, M. Curie Skłodowskiej 10C Str., 41-800 Zabrze, Poland.
Chair and Department of Oral Surgery, Medical University of Lublin, Chodźki 6 Str., 20-093 Lublin, Poland.
Microorganisms. 2021 Nov 15;9(11):2359. doi: 10.3390/microorganisms9112359.
The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at the same time, avoiding increasing the surface roughness during decontamination) the effects of diode laser doses were investigated. The irradiation was performed with a diode laser at the wavelength of λ = 810 nm in three variants with a different number of repetitions (1 × 15 s, 2 × 15 s, 3 × 15 s). The quantitative microbial contamination of the surface of teeth, porcelain and zirconia crowns assessment was made using the culture-dependent method. The identification of microorganisms took place using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and next-generation sequencing (NGS) methods. The studies of the surface morphology and roughness were carried out by means of the optical profilometry, scanning electron microscopy (SEM) and optical microscopy with the C1 confocal attachment. The most important conclusion from the research is the fact that the laser operation, regardless of the exposure time, effectively eliminates the microorganisms from the surfaces used for dental implant rebuilding and does not have a destructive effect on the tested material.
本文的目的是研究二极管激光（LD）去除种植义齿中使用的瓷和氧化锆冠表面分离出的微生物的有效性，以尽量减少牙种植体周围的感染。为了优化该过程的杀菌效果（同时避免在去污过程中增加表面粗糙度），研究了二极管激光剂量的影响。使用波长为λ = 810 nm的二极管激光进行照射，分为三种不同重复次数的变体（1×15秒、2×15秒、3×15秒）。使用依赖培养的方法对牙齿、瓷和氧化锆冠表面的定量微生物污染进行评估。使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）和下一代测序（NGS）方法对微生物进行鉴定。通过光学轮廓测量、扫描电子显微镜（SEM）和带有C1共聚焦附件的光学显微镜对表面形态和粗糙度进行研究。该研究最重要的结论是，无论暴露时间如何，激光操作都能有效消除用于牙种植体重建的表面上的微生物，并且对测试材料没有破坏作用。
