Wawrzyk Anna, Dymel Marzena, Guzińska Krystyna, Cywiński Piotr, Papis Aleksandra, Konka Adam, Wawrzyk-Bochenek Iga, Wilczyński Sławomir
Auschwitz-Birkenau State Museum, Więźniów Oświęcimia 20, 32-603 Oświęcim, Poland.
Silesian Park of Medical Technology Kardio-Med Silesia in Zabrze, M. Curie Skłodowskiej 10C Str., 41-800 Zabrze, Poland.
Materials (Basel). 2023 Mar 28;16(7):2700. doi: 10.3390/ma16072700.
The aim of the study was to assess the biocidal effectiveness and the effect of 80% and 90% ethanol applied in the form of mist on the surface of textile materials from historical A-BSM objects. The microorganisms used for the tests, namely, , and , were isolated from the surface of textile objects in the A-BSM. , , and were also used from the American Type Culture Collection (ATCC). Fabric samples were inoculated with microorganisms at a concentration of 10-10 CFU/ml. Ethanol in the form of mist was applied in concentrations of 80% and 90%. Airbrushes VL 0819 and VE 0707 were used for this purpose, where the pressure was 0.2 MPa and the PA HEAD VLH-5 nozzle with a tip of 1.05 mm in diameter was used. In order to achieve more effective disinfection after applying the ethanol mist, samples were stored in PE foil in the conditions of 21 °C ± 1 °C for 22 ± 1 h. After applying the ethanol mist, changes in the properties of the materials were assessed using scanning electron microscopy (SEM). The reduction in the number of microorganisms on modern cotton fabric after the use of ethanol in the form of mist at concentrations of 80% and 90% ranged from 93.27% to 99.91% for fungi and from 94.96% to 100% for bacteria, except for 74.24% for . On the historical fabric, after the time of application of 90% ethanol was shortened to 4 s, the microorganisms were reduced by over 99.93% and was completely eliminated. After applying the tested disinfection technique, no changes in fiber morphology were observed on the surface of the model and historical cotton.
本研究的目的是评估以雾状形式施加的80%和90%乙醇对历史A-BSM物品纺织材料表面的杀菌效果及影响。用于测试的微生物，即 、 和 ，是从A-BSM纺织物品表面分离出来的。 、 、 和 也取自美国典型培养物保藏中心（ATCC）。织物样品接种微生物的浓度为10-10 CFU/ml。以雾状形式施加的乙醇浓度为80%和90%。为此使用了VL 0819和VE 0707喷枪，压力为0.2 MPa，使用直径为1.05 mm尖端的PA HEAD VLH-5喷嘴。为了在施加乙醇雾后实现更有效的消毒，样品在21 °C ± 1 °C条件下用PE箔保存22 ± 1 h。施加乙醇雾后，使用扫描电子显微镜（SEM）评估材料性能的变化。在现代棉织物上，使用浓度为80%和90%的雾状乙醇后，真菌数量减少93.27%至99.91%，细菌数量减少94.96%至100%，但 除外，其减少率为74.24%。在历史织物上，将90%乙醇的施加时间缩短至4 s后，微生物减少超过99.93%， 被完全消除。应用测试的消毒技术后，在模型棉和历史棉的表面未观察到纤维形态的变化。
