Smith R F, King K Y
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Protein Sci. 1995 Jan;4(1):126-9. doi: 10.1002/pro.5560040115.
Primary sequence patterns based on known conserved sites in eukaryotic protein kinases were used to search for eukaryotic-like protein kinase sequences in a six-frame translation of the bacterial subsection of GenBank. This search identified a previously unrecognized eukaryotic-like protein kinase gene in three related methanogenic archaebacteria, Methanococcus vannielii, M. voltae, and M. thermolithotrophicus. The proposed coding sequences are located in orthologous open reading frames (ORFs): ORF547, ORF294, and ORF114, respectively. The C-terminus of the ORFs contains 9 of the 11 subdomains characteristically conserved within the eukaryotic protein kinase catalytic domain. The N-terminus of the ORFs is similar to a putative glycoprotease in Pasteurella haemolytica and its homologue in Escherichia coli, the orfX gene. This is the first report of a eukaryotic-like protein kinase sequence observed in Archaebacteria.
基于真核生物蛋白激酶中已知保守位点的一级序列模式，用于在GenBank细菌部分的六框架翻译中搜索类真核生物蛋白激酶序列。该搜索在三种相关的产甲烷古细菌——万氏甲烷球菌、沃氏甲烷球菌和嗜热嗜石甲烷球菌中鉴定出一个以前未被识别的类真核生物蛋白激酶基因。拟编码序列分别位于直系同源开放阅读框（ORF）中：ORF547、ORF294和ORF114。这些ORF的C末端包含真核生物蛋白激酶催化域内特征性保守的11个亚结构域中的9个。这些ORF的N末端类似于溶血巴斯德氏菌中的一种假定糖蛋白酶及其在大肠杆菌中的同源物orfX基因。这是在古细菌中观察到类真核生物蛋白激酶序列的首次报道。
