Faculty of Chemistry, Bioanalysis Laboratory, University of Bialystok, Ciolkowskiego 1K, 15-245, Bialystok, Poland.
Faculty of Chemical Technology, Poznan University of Technology, Pl. Sklodowskiej-Curie 5, 60-965, Poznan, Poland.
Anal Biochem. 2020 Nov 15;609:113964. doi: 10.1016/j.ab.2020.113964. Epub 2020 Sep 23.
Carcinoembryonic antigen (CEA) is one of the biomarkers most commonly used to determine tumor activity. In this work, a Surface Plasmon Resonance imaging (SPRi) immunosensor was developed. The immunosensor consists of a cysteamine linker attached to a gold chip and mouse monoclonal anti-CEA antibody bonded by the "EDC/NHS protocol". The formation of successive immunosensor layers was confirmed by AFM measurements. The concentration of the antibody was optimized. The linear response range of the developed immunosensor is between 0.40 and 20 ng mL, and it is suitable for CEA measurement in both blood cancer patients and healthy individuals. Only 3 μL of serum or plasma sample is required, and no preconcentration is used. The method has a precision of 2-16%, a recovery of 101-104% depending on CEA concentration, a detection limit of 0.12 ng mL and a quantification limit of 0.40 ng mL. The method is selective (with respect to albumin, leptin, interleukin 6, metalloproteinase-1, metallopeptidase inhibitor 1 and CA 125/MUC16) and it was validated by comparison with the standard electrochemiluminescence method on a series of colorectal cancer blood samples.
癌胚抗原（CEA）是最常用于确定肿瘤活性的生物标志物之一。在这项工作中，开发了一种表面等离子体共振成像（SPRi）免疫传感器。该免疫传感器由连接到金芯片的半胱氨酸接头和通过“EDC/NHS 方案”键合的小鼠单克隆抗 CEA 抗体组成。通过 AFM 测量确认了连续免疫传感器层的形成。优化了抗体的浓度。所开发的免疫传感器的线性响应范围在 0.40 和 20ng mL 之间，适用于血液癌症患者和健康个体的 CEA 测量。仅需要 3μL 的血清或血浆样品，并且不使用预浓缩。该方法的精度为 2-16%，回收率为 101-104%，具体取决于 CEA 浓度，检测限为 0.12ng mL，定量限为 0.40ng mL。该方法具有选择性（相对于白蛋白、瘦素、白细胞介素 6、金属蛋白酶 1、金属肽酶抑制剂 1 和 CA 125/MUC16），并通过与一系列结直肠癌血液样本的标准电化学发光法进行比较进行了验证。
