Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, South Korea.
Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, South Korea.
Int J Biol Macromol. 2020 Dec 15;165(Pt A):747-757. doi: 10.1016/j.ijbiomac.2020.09.177. Epub 2020 Sep 24.
The present work synthesized nano-chitosan capped gold nanoparticles (CS-AuNPs) and tested its bioactivities. The fourier-transform infrared spectroscopy (FTIR) exhibited glucosamine group revealed the capping of chitosan in CS-AuNPs. The X-ray photoelectron spectroscopy (XPS) demonstrated the peaks at 84.49 eV and 88.49 eV was corresponding to gold. The dynamic light scattering (DLS) analysis indicated the size of 98.14 ± 6.62 nm, poly dispersion index (PDI) of 0.232 and zeta potential of 46.8 ± 2.07 mV for CS-AuNPs. The CS-AuNPs was exhibited the higher antioxidant, antibacterial and diabetes related enzyme inhibitory activities than that in CSNPs. The 50% of LN229 cell death was occurred through the treatment of 218.75 μg. mL and 500 μg. mL of CS-AuNPs and CSNPs respectively. The inhibitory concentration (IC) of CS-AuNPs showed higher cytotoxicity in LN229 via cellular damage, reactive oxygen species (ROS), and arresting about 42.33% cells in the G phase. These results proved the multifunctional bioactive properties of CS-AuNPs.
本研究合成了纳米壳聚糖包裹的金纳米粒子（CS-AuNPs），并测试了其生物活性。傅里叶变换红外光谱（FTIR）显示了壳聚糖中的氨基葡萄糖基团，表明壳聚糖在 CS-AuNPs 中被包裹。X 射线光电子能谱（XPS）表明，84.49 eV 和 88.49 eV 的峰分别对应于金。动态光散射（DLS）分析表明 CS-AuNPs 的粒径为 98.14 ± 6.62nm，多分散指数（PDI）为 0.232，zeta 电位为 46.8 ± 2.07 mV。CS-AuNPs 表现出比 CSNPs 更高的抗氧化、抗菌和糖尿病相关酶抑制活性。通过处理 218.75 μg.mL 和 500 μg.mL 的 CS-AuNPs 和 CSNPs，LN229 细胞的 50%死亡。CS-AuNPs 的抑制浓度（IC）在 LN229 中通过细胞损伤、活性氧（ROS）显示出更高的细胞毒性，使大约 42.33%的细胞停滞在 G 期。这些结果证明了 CS-AuNPs 的多功能生物活性特性。
