Clinic of Dermatology, University Hospital of Aachen, Aachen 52074, Germany; Department of Pharmacology, Medical Research Division, National Research Center, Dokki, Cairo 12622, Egypt.
Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk, Gdansk, Poland.
Carbohydr Polym. 2021 Mar 15;256:117498. doi: 10.1016/j.carbpol.2020.117498. Epub 2020 Dec 14.
The present work describes the synthesis of a new series of chitosan-gold hybrid nanoparticles (CS-AuNPs) for the delivery of Punicagranatum L. extract (PE). It proposes CS and PE as reducing agents for gold ions in aqueous solution. The effect of PE on the physicochemical properties of the CS-AuNPs was investigated with UV spectroscopy, DLS, DSC, XRD, FTIR, SEM/EDX and TEM. Interestingly, about 50 % reduction in size was observed with using PE alone for gold reduction. The ζ-potential of CS-AuNPs was shifted from +53.1 ± 6.7 mV to 31.0 ± 6.0 mV upon conjugation of the negatively-charged PE polyphenols. The developed PE-conjugated CS-AuNPs exhibited higher stability at different pH values. About 87 % of the loaded PE was released from the NPs over 24 h. The antibacterial activity of CS-PE-AuNPs displayed a synergetic affect against methicillin-resistant S. aureus with MIC and MBC values of 15.6 and 62.5 μg/mL, respectively.
本工作描述了一系列新型壳聚糖-金杂化纳米粒子（CS-AuNPs）的合成，用于递送石榴皮提取物（PE）。它提出了 CS 和 PE 作为水溶液中金离子的还原剂。通过紫外光谱、DLS、DSC、XRD、FTIR、SEM/EDX 和 TEM 研究了 PE 对 CS-AuNPs 物理化学性质的影响。有趣的是，单独使用 PE 还原金时，观察到尺寸约减少了 50%。PE 上的带负电荷多酚与 CS-AuNPs 结合后，ζ-电势从+53.1±6.7 mV 变为+31.0±6.0 mV。所开发的 PE 缀合 CS-AuNPs 在不同 pH 值下表现出更高的稳定性。在 24 小时内，约 87%的负载 PE 从 NPs 中释放出来。CS-PE-AuNPs 的抗菌活性对耐甲氧西林金黄色葡萄球菌表现出协同作用，其 MIC 和 MBC 值分别为 15.6 和 62.5 μg/mL。
