Lingwood C A, Law H, Richardson S, Petric M, Brunton J L, De Grandis S, Karmali M
J Biol Chem. 1987 Jun 25;262(18):8834-9.
Escherichia coli verotoxin (also known as Shiga-like toxin) has been implicated in the aetiology of the hemolytic uremic syndrome and hemorrhagic colitis. The glycolipid binding specificity of verotoxin purified from E. coli H30 and verotoxin cloned from bacteriophage H19B has been examined. Verotoxin from both sources binds specifically to globotriosyl ceramide containing the carbohydrate sequence galactose alpha 1-4galactose beta 1-4glucose-ceramide. Removal of the terminal galactose or substitution with N-acetylgalactosamine in beta 1-3 linkage deletes toxin binding activity. A ceramide trihexoside species, consistent with a globotriosyl ceramide structure was shown to be the major verotoxin-binding glycolipid of cultured vero cells which are routinely used to measure the cytotoxicity of toxin samples.
大肠杆菌维罗毒素（也称为志贺样毒素）与溶血性尿毒症综合征和出血性结肠炎的病因有关。对从大肠杆菌H30纯化的维罗毒素以及从噬菌体H19B克隆的维罗毒素的糖脂结合特异性进行了研究。来自这两种来源的维罗毒素都特异性结合含有碳水化合物序列半乳糖α1-4半乳糖β1-4葡萄糖-神经酰胺的球三糖基神经酰胺。去除末端半乳糖或以β1-3连接的N-乙酰半乳糖胺替代会消除毒素结合活性。一种与球三糖基神经酰胺结构一致的神经酰胺三己糖苷被证明是培养的非洲绿猴肾细胞的主要维罗毒素结合糖脂，这些细胞通常用于测量毒素样品的细胞毒性。
