Weil G J, Jain D C, Santhanam S, Malhotra A, Kumar H, Sethumadhavan K V, Liftis F, Ghosh T K
J Infect Dis. 1987 Aug;156(2):350-5. doi: 10.1093/infdis/156.2.350.
We evaluated a monoclonal antibody-based enzyme immunoassay for detecting soluble parasite antigen in sera collected in an area in South India endemic for Wuchereria bancrofti. Filarial antigen was detected in sera from 56 of 57 microfilaremic patients, 9 of 64 aminofilaremic patients with clinical filariasis, and 11 of 70 endemic controls. Antigen was not detected in sera from patients from nonendemic areas who had a variety of other filarial and nonfilarial helminth infections. Parasite antigen titers were significantly correlated with microfilarial counts in night blood smears (r = .64, P less than .01). Negative antigen tests in patients with clinical filariasis may be explained in part by antibody-mediated clearance of circulating antigen. Antibodies to circulating W. bancrofti antigen were detected in 41 of 55 antigen-negative sera from patients with clinical filariasis. Despite this limitation, detecting parasite antigen by enzyme immunoassay provides significant advantages over previously available methods for diagnosing active W. bancrofti infection.
我们评估了一种基于单克隆抗体的酶免疫测定法，用于检测在印度南部班氏吴策线虫流行地区采集的血清中的可溶性寄生虫抗原。在57例微丝蚴血症患者中的56例、64例有临床丝虫病的无丝蚴血症患者中的9例以及70例地方性对照中的11例血清中检测到了丝虫抗原。在来自非流行地区、患有各种其他丝虫和非丝虫蠕虫感染的患者血清中未检测到抗原。寄生虫抗原滴度与夜间血涂片微丝蚴计数显著相关（r = 0.64，P < 0.01）。临床丝虫病患者抗原检测阴性部分原因可能是抗体介导的循环抗原清除。在55例临床丝虫病患者的抗原阴性血清中有41例检测到了针对循环班氏吴策线虫抗原的抗体。尽管有此局限性，但通过酶免疫测定法检测寄生虫抗原比以前用于诊断活动性班氏吴策线虫感染的方法具有显著优势。
