Ramzy R M, Gad A M, Faris R, Weil G J
Research and Training Center on Vectors of Diseases, Ain Shams University, Cairo, Egypt.
Am J Trop Med Hyg. 1991 Jun;44(6):691-5. doi: 10.4269/ajtmh.1991.44.691.
Conventional methods for diagnosis of Wuchereria bancrofti infection are insensitive and often impractical because of the need for night blood collections. A sensitive and specific antigen detection assay has been developed for W. bancrofti, which is based on a monoclonal antibody (AD12) that binds to a repeated epitope on a 200 kDa adult worm excretion product present in sera from infected humans. The only formal evaluation of this assay to date was performed with sera from India. In the present study, we have evaluated the performance of the AD12 antigen assay in two laboratories with sera collected in endemic and non-endemic areas in Egypt. Antigen was detected in 57 of 59 (97%) sera from microfilaremic subjects, and in 22 of 139 asymptomatic and amicrofilaremic subjects who reside in a highly endemic area. Antigen titers were significantly correlated with microfilaria counts (r = 0.41, P less than 0.01). Filarial antigen was not detected in most sera from amicrofilaremic subjects with clinical filariasis. Comparative antigen test results obtained from laboratories in Cairo and St. Louis agreed in 170 of 173 sera tested. Filarial antigen was not detected in sera from Egyptians with no history of residence in filaria-endemic areas. Specifically, nonendemic sera from patients with other parasitic infections (schistosomiasis, fascioliasis, ascariasis, etc.) were uniformly negative in the assay. We conclude that the AD12 filarial antigen assay is sensitive and specific for W. bancrofti infection in Egypt.
由于需要在夜间采集血液，班氏吴策线虫感染的传统诊断方法灵敏度较低且通常不实用。现已开发出一种针对班氏吴策线虫的灵敏且特异的抗原检测方法，该方法基于一种单克隆抗体（AD12），它能与感染人类血清中存在的200 kDa成虫排泄产物上的重复表位结合。迄今为止，对该检测方法的唯一正式评估是使用来自印度的血清进行的。在本研究中，我们在两个实验室中用在埃及流行区和非流行区采集的血清评估了AD12抗原检测方法的性能。在59名微丝蚴血症患者的57份血清（97%）中检测到了抗原，在居住于高度流行区的139名无症状且无微丝蚴血症患者的22份血清中也检测到了抗原。抗原滴度与微丝蚴计数显著相关（r = 0.41，P < 0.01）。在大多数患有临床丝虫病的无微丝蚴血症患者的血清中未检测到丝虫抗原。开罗和圣路易斯实验室对173份血清进行的比较抗原检测结果在170份血清中一致。在无丝虫病流行区居住史的埃及人的血清中未检测到丝虫抗原。具体而言，患有其他寄生虫感染（血吸虫病、肝片吸虫病、蛔虫病等）患者的非流行区血清在该检测中均为阴性。我们得出结论，AD12丝虫抗原检测方法对埃及的班氏吴策线虫感染灵敏且特异。
