Meyer R J, Helinski D R
Biochim Biophys Acta. 1977 Sep 6;478(1):109-13. doi: 10.1016/0005-2787(77)90249-0.
The mode of replication of the broad host-range plasmid RK2 has been determined from examination of molecular replicative forms cleaved with the restriction endonucleases EcoRI and Hind III. Replication is unidirectional, and proceeds from a unique origin. The location of the origin and other evidence suggests that genes involved in plasmid maintenance are not tightly clustered.
通过对用限制性内切酶EcoRI和Hind III切割的分子复制形式进行检测，已确定了广宿主范围质粒RK2的复制模式。复制是单向的，且从一个独特的起点开始。起点的位置及其他证据表明，参与质粒维持的基因并非紧密聚集。
