Meyer R, Figurski D, Helinski D R
Mol Gen Genet. 1977 Apr 29;152(3):129-35. doi: 10.1007/BF00268809.
The cleavage map of the plasmid RK2 was determined for the five restriction endonucleases EcoRI, HindIII, BamH-I, SalI and HpaI. DNA has been inserted into several of these sites and cloned in Escherichia coli. Efforts to obtain derivatives of RK2 reduced in size by restriction endonuclease digestion of the plasmid were not successful and indicated that genes required for the maintenance of this plasmid in E. coli are not tightly clustered. An RK2 derivative possessing an internal molecular rearrangement was obtained by transformation with restriction endonuclease digests of the plasmid.
确定了质粒RK2对于五种限制性内切核酸酶EcoRI、HindIII、BamH-I、SalI和HpaI的切割图谱。DNA已插入其中几个位点并在大肠杆菌中克隆。通过对质粒进行限制性内切核酸酶消化来获得尺寸减小的RK2衍生物的尝试未成功，这表明该质粒在大肠杆菌中维持所需的基因并非紧密聚集。通过用质粒的限制性内切核酸酶消化产物进行转化，获得了一种具有内部分子重排的RK2衍生物。
