Guo Wei, Yin Gaofei, Liu Hongfei, Duan Hanyuan, Huang Zhigang
Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China.
Oncol Lett. 2020 Nov;20(5):271. doi: 10.3892/ol.2020.12134. Epub 2020 Sep 21.
The aim of the present study was to analyze the characteristics of angiogenic factors in patients with hypopharyngeal cancer, and to study the effects of these factors on induction chemotherapy, patient prognosis and survival. Data from 60 eligible patients with hypopharyngeal cancer that were treated between January 2012 and December 2016 were collected retrospectively. The differential expression of angiogenic factors in tumor and peritumoral tissues was analyzed retrospectively to assess the association between five differentially expressed genes, including interleukin (IL)-1β, transforming growth factor (TGF)-β, matrix metalloproteinase-9 (MMP-9), angiopoietin-2 and interferon-inducible T-cell α chemoattractant, and clinicopathological characteristics in different types of chemotherapy-associated blood vessels within samples of poorly differentiated hypopharyngeal cancer. The χ test or t-test was used to compare the frequency data, the Kaplan-Meier method was used for survival analysis and the log-rank test was used to compare the Kaplan-Meier curves. P<0.05 was considered to indicate a statistically significant difference. The results of the present study demonstrated that there was a significant difference in the expression levels of vascular-associated factors between hypopharyngeal carcinoma and peritumoral tissues. Additionally, the results revealed a significant difference in the overall survival and prognosis of patients with a decreased vascular classification compared with patients with an unchanged vascular classification, which was assessed using narrowband imaging (NBI) following induction chemotherapy (P<0.05). The results of single factor analysis indicated that IL-1β, TGF-β and MMP-9 were associated with decreased blood vessel classification (P<0.05). In conclusion, IL-1β, TGF-β and MMP-9 may be used as predictors of the effect of induction chemotherapy on poorly differentiated hypopharyngeal cancer. Therefore, when patients with advanced hypopharyngeal cancer undergo chemotherapy, NBI vascular examination and screening for associated vascular factors should be performed before and after chemotherapy. Alterations in vascular classification, assessed using NBI, and abnormal expression of vascular factors may also be used as reference factors for prognosis.
本研究旨在分析下咽癌患者血管生成因子的特征，并研究这些因子对诱导化疗、患者预后和生存的影响。回顾性收集了2012年1月至2016年12月期间接受治疗的60例符合条件的下咽癌患者的数据。回顾性分析肿瘤组织和瘤周组织中血管生成因子的差异表达，以评估包括白细胞介素（IL）-1β、转化生长因子（TGF）-β、基质金属蛋白酶-9（MMP-9）、血管生成素-2和干扰素诱导的T细胞α趋化因子在内的5个差异表达基因与低分化下咽癌样本中不同类型化疗相关血管的临床病理特征之间的关联。采用χ检验或t检验比较频率数据，采用Kaplan-Meier法进行生存分析，采用对数秩检验比较Kaplan-Meier曲线。P<0.05被认为具有统计学显著差异。本研究结果表明，下咽癌组织与瘤周组织中血管相关因子的表达水平存在显著差异。此外，结果显示，与诱导化疗后使用窄带成像（NBI）评估的血管分级未改变的患者相比，血管分级降低的患者的总生存期和预后存在显著差异（P<0.05）。单因素分析结果表明，IL-1β、TGF-β和MMP-9与血管分级降低有关（P<0.05）。总之，IL-1β、TGF-β和MMP-9可作为诱导化疗对低分化下咽癌疗效的预测指标。因此，晚期下咽癌患者化疗时，应在化疗前后进行NBI血管检查及相关血管因子筛查。NBI评估的血管分级改变及血管因子异常表达也可作为预后的参考因素。
