Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo, China.
Wuzhi Sub-Bureau of Jiaozuo City Ecological Environment Bureau, Wuzhi, China.
Curr Microbiol. 2020 Dec;77(12):3909-3918. doi: 10.1007/s00284-020-02219-5. Epub 2020 Sep 28.
Wastewater (WW) irrigation to agricultural soils is one of the most economical and effective water-saving strategies. The effects of WW irrigation on soil microbial communities have gained increasing focus as these effects are not well understood. In this study, the effects of WW and groundwater (GW) irrigation on microbial diversity and structure were compared using the high-throughput sequencing analysis of 16S rDNA amplicons. Soil samples irrigated by WW for several decades and maize soil (loamy) samples irrigated by GW were collected from Luancheng Town, Shijiazhuang City, China. Compared to the GW groups, WW groups exhibited non-significant soil bacterial community abundance at the 0-20 and 20-40 cm depths. WW irrigation significantly altered the bacterial community composition and structures compared to GW irrigation. The relative abundance of Proteobacteria and Firmicutes increased in WW irrigated soil, while Actinobacteria decreased. Moreover, 14 significantly abundant biomarkers from Proteobacteria and Firmicutes that corresponded with WW irrigation were identified. Additionally, WW irrigation enriched some KEGG pathways that corresponded with metabolism and human diseases. The physical and chemical properties of WW irrigated soil may shape the compositions and structures of soil bacterial communities. The findings of this study illuminated the effects of wastewater irrigation on microbial characteristics, which is important for estimating the effects of long-term wastewater irrigation on soil environmental health.
污水（WW）灌溉农业土壤是最经济和有效的节水策略之一。由于这些影响尚未得到很好的理解，因此 WW 灌溉对土壤微生物群落的影响越来越受到关注。在这项研究中，使用高通量测序分析 16S rDNA 扩增子比较了 WW 和地下水（GW）灌溉对微生物多样性和结构的影响。从中国石家庄市栾城镇采集了几十年灌溉 WW 的土壤样本和灌溉 GW 的玉米土壤（壤土）样本。与 GW 组相比，WW 组在 0-20 和 20-40 cm 深度的土壤细菌群落丰度没有显著差异。与 GW 灌溉相比，WW 灌溉显著改变了细菌群落组成和结构。与 GW 灌溉相比，WW 灌溉土壤中变形菌门和厚壁菌门的相对丰度增加，放线菌门减少。此外，还鉴定出与 WW 灌溉相对应的来自变形菌门和厚壁菌门的 14 个显著丰富的生物标志物。此外，WW 灌溉还丰富了一些与代谢和人类疾病相对应的 KEGG 途径。WW 灌溉土壤的物理和化学性质可能会影响土壤细菌群落的组成和结构。本研究的结果阐明了废水灌溉对微生物特征的影响，这对于估计长期废水灌溉对土壤环境健康的影响非常重要。
