Vlasov V V, Knorre D G, Skobel'tsyna L M
Biokhimiia. 1977 May;42(5):784-7.
The influence of alkylation of phe-tRNAPhe (E. coli) with 2',3'-O-[4-(N-2-chloroethyl-N-methylamino)-benzylidene]-uridine - 5' - methylphosphate on its ability to participate in non-enzymatic complex formation with ribosomes and poly-U was investigated. Phe-tRNAsPhe, containing alkylated guanosines at different positions, including anticodone, are active in binding with ribosomes. It is concluded, that N7 nitrogens of guanosine of the tRHAPhe are not elements, significant for the interaction with ribosomes.
研究了用2',3'-O-[4-(N-2-氯乙基-N-甲基氨基)-亚苄基]-尿苷-5'-甲基磷酸对苯丙氨酸-tRNA苯丙氨酸（大肠杆菌）进行烷基化修饰后，其参与与核糖体和聚尿苷酸形成非酶复合物能力的影响。在不同位置（包括反密码子）含有烷基化鸟苷的苯丙氨酸-tRNA苯丙氨酸在与核糖体结合方面具有活性。得出的结论是，tRNA苯丙氨酸中鸟苷的N7氮原子不是与核糖体相互作用的重要元素。
