CSIR- Central Scientific Instruments Organisation, Sector 30-C, Chandigarh -160030, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad -201002, India.
ACS Appl Mater Interfaces. 2020 Oct 21;12(42):48198-48205. doi: 10.1021/acsami.0c14312. Epub 2020 Oct 9.
Metal-organic frameworks (MOFs) are envisaged as highly useful for the development of biosensors. Herein, for the first time, we report the optical detection of using a water-dispersible terbium MOF (Tb-BTC; BTC, 1,3,5-benzenetricarboxylic acid). The successful synthesis of Tb-BTC is verified using spectroscopic and morphological techniques like UV-vis, fluorescence and FTIR spectroscopy, X-ray diffraction analysis, and electron microscopy. Tb-BTC has been bio-interfaced with anti- antibodies and then investigated as a biosensor for . The biosensor displays detection ability in an analyte concentration range of 1.3 × 10 to 1.3 × 10 cfu/mL with a detection limit of 3 cfu/mL, having a response time of 5 min and a total analysis time of about 20-25 min. The results are also found to be reproducible and specific in the presence of some other interfering bacterial species. As demonstrated, the present sensor provides highly sensitive and specific detection of in fruit juice sample. To the best of our knowledge, this is the first report to showcase the potential of the MOF-based fluorescent biosensor for the detection of .
金属-有机骨架（MOFs）被认为在生物传感器的开发中非常有用。在此，我们首次报道了使用水可分散的铽 MOF（Tb-BTC；BTC，1,3,5-苯三甲酸）对进行光学检测。通过光谱和形态学技术（如 UV-vis、荧光和傅里叶变换红外光谱、X 射线衍射分析和电子显微镜）验证了 Tb-BTC 的成功合成。Tb-BTC 已与抗 抗体进行生物接口化，然后作为 生物传感器进行研究。该生物传感器在 1.3×10 到 1.3×10 cfu/mL 的分析物浓度范围内显示出检测能力，检测限为 3 cfu/mL，响应时间为 5 分钟，总分析时间约为 20-25 分钟。在存在一些其他干扰细菌种类的情况下，结果也被发现具有重现性和特异性。如所展示的，本传感器提供了果汁样品中 高度敏感和特异性的检测。据我们所知，这是第一个展示基于 MOF 的荧光生物传感器用于检测 的报告。
