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食品源性致病菌的光谱检测方法研究进展。

Recent Progress in Spectroscopic Methods for the Detection of Foodborne Pathogenic Bacteria.

机构信息

School of Materials and Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, China.

Postdoctoral Innovation Practice, Shenzhen Polytechnic, Liuxian Avenue, Nanshan District, Shenzhen 518055, China.

出版信息

Biosensors (Basel). 2022 Oct 13;12(10):869. doi: 10.3390/bios12100869.

Abstract

Detection of foodborne pathogens at an early stage is very important to control food quality and improve medical response. Rapid detection of foodborne pathogens with high sensitivity and specificity is becoming an urgent requirement in health safety, medical diagnostics, environmental safety, and controlling food quality. Despite the existing bacterial detection methods being reliable and widely used, these methods are time-consuming, expensive, and cumbersome. Therefore, researchers are trying to find new methods by integrating spectroscopy techniques with artificial intelligence and advanced materials. Within this progress report, advances in the detection of foodborne pathogens using spectroscopy techniques are discussed. This paper presents an overview of the progress and application of spectroscopy techniques for the detection of foodborne pathogens, particularly new trends in the past few years, including surface-enhanced Raman spectroscopy, surface plasmon resonance, fluorescence spectroscopy, multiangle laser light scattering, and imaging analysis. In addition, the applications of artificial intelligence, microfluidics, smartphone-based techniques, and advanced materials related to spectroscopy for the detection of bacterial pathogens are discussed. Finally, we conclude and discuss possible research prospects in aspects of spectroscopy techniques for the identification and classification of pathogens.

摘要

早期检测食源性病原体对于控制食品质量和改善医疗反应非常重要。快速、高灵敏度和特异性地检测食源性病原体成为健康安全、医学诊断、环境安全和控制食品质量方面的迫切要求。尽管现有的细菌检测方法可靠且应用广泛,但这些方法耗时、昂贵且繁琐。因此,研究人员试图通过将光谱技术与人工智能和先进材料相结合来寻找新方法。在本进展报告中,讨论了利用光谱技术检测食源性病原体的最新进展。本文综述了光谱技术在食源性病原体检测中的应用和进展,特别是过去几年中的新趋势,包括表面增强拉曼光谱、表面等离子体共振、荧光光谱、多角度激光光散射和成像分析。此外,还讨论了与光谱学检测细菌病原体相关的人工智能、微流控、基于智能手机的技术和先进材料的应用。最后,我们从光谱技术在病原体识别和分类方面的角度总结并讨论了可能的研究前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62eb/9599795/c9bc5eb9acb8/biosensors-12-00869-g001.jpg

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