Eisenstark A, Perrot G
Mol Gen Genet. 1987 Apr;207(1):68-72. doi: 10.1007/BF00331492.
In bacterial cells near-ultraviolet radiation (NUV) generates H2O2 which can be decomposed by endogenous catalase to H2O and O2. To assess the roles of H2O2 and catalase in NUV lethality, we manipulated the amount of intracellular catalase (a) by the use of mutant and plasmid strains with altered endogenous catalase, (b) physiologically, by the addition of glucose, and (c) by induction of catalase synthesis with oxidizing agents. Not only was there no direct correlation between NUV-resistance and catalase activity, but in some cases the correlation was inverse. Also, while there was correlation between NUV and H2O2 sensitivity for most strains tested, there were a number of exceptions which indicates that the modes of killing were different for the two agents.
在细菌细胞中，近紫外线辐射（NUV）会产生过氧化氢，而过氧化氢可被内源性过氧化氢酶分解为水和氧气。为了评估过氧化氢和过氧化氢酶在NUV致死性中的作用，我们通过以下方式操纵细胞内过氧化氢酶的量：（a）使用内源性过氧化氢酶改变的突变体和质粒菌株；（b）通过添加葡萄糖进行生理调节；（c）用氧化剂诱导过氧化氢酶合成。不仅NUV抗性与过氧化氢酶活性之间没有直接相关性，而且在某些情况下相关性是相反的。此外，虽然大多数测试菌株的NUV敏感性与过氧化氢敏感性之间存在相关性，但也有许多例外情况，这表明两种试剂的杀伤模式不同。
