大豆异黄酮染料木黄酮通过调节高脂饮食诱导的糖尿病小鼠的胰岛素信号通路来减轻胰岛素抵抗。
Biochanin A, a soy isoflavone, diminishes insulin resistance by modulating insulin-signalling pathway in high-fat diet-induced diabetic mice.
作者信息
Arjunan Sundaresan, Thangaiyan Radhiga, Balaraman Deivasigamani
机构信息
CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, India.
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India.
出版信息
Arch Physiol Biochem. 2023 Apr;129(2):316-322. doi: 10.1080/13813455.2020.1820525. Epub 2020 Sep 29.
AIM
The objective of this study was to evaluate the probable mechanism of action of Biochanin A (BCA) on high-fat diet-induced insulin resistance mice.
METHODS
Twenty-four male C57/BL/6J mice were divided into two and fed either control diet or a high fat-high diet (HFD). After 6 weeks, mice were grouped into: Control, Control + BCA, HFD and HFD + BCA ( = 6). Mice were made diabetic by feeding them an HFD.
RESULTS
Body weight, plasma glucose, insulin, leptin levels and HOMA values were significantly lower in the BCA supplemented HFD group when related to the HFD group. Furthermore, BCA supplementation significantly increased Insulin receptor substrate 1 (IRS 1), PI3K, Akt and Glucose transporter type 4 (GLUT-4) protein abundance in skeletal muscle when equated with HFD.
CONCLUSION
This study demonstrates that BCA can improve insulin sensitivity by activating insulin signalling, suggesting that it may possess antidiabetic activities.
目的
本研究的目的是评估染料木黄酮（BCA）对高脂饮食诱导的胰岛素抵抗小鼠的可能作用机制。
方法
将24只雄性C57/BL/6J小鼠分为两组，分别喂食对照饮食或高脂高糖饮食（HFD）。6周后，小鼠被分为：对照组、对照+BCA组、HFD组和HFD+BCA组（每组=6只）。通过喂食HFD使小鼠患糖尿病。
结果
与HFD组相比，补充BCA的HFD组的体重、血糖、胰岛素、瘦素水平和HOMA值显著降低。此外，与HFD组相比，补充BCA显著增加了骨骼肌中胰岛素受体底物1（IRS 1）、PI3K、Akt和葡萄糖转运蛋白4（GLUT-4）的蛋白丰度。
结论
本研究表明，BCA可通过激活胰岛素信号改善胰岛素敏感性，提示其可能具有抗糖尿病活性。
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