Division of Applied Phycology and Biotechnology, CSIR-Central Salt and Marine Chemicals Research Institute, GB Marg, Bhavnagar, Gujarat, 364002, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Mol Biol Rep. 2020 Oct;47(10):7403-7411. doi: 10.1007/s11033-020-05795-8. Epub 2020 Sep 29.
Seaweeds are considered a promising functional food and safe for human consumption as they have significant health benefits. Five abundant tropical seaweeds, Caulerpa racemosa var. macrophysa, Caulerpa scalpelliformis, Grateloupia indica, Sargassum linearifolium, and Spatoglossum asperum rich in metabolites, phenolic, and flavonoid compounds, were analyzed for the anti-proliferative and ROS inhibitory activities including transcript expression of cancer-linked key genes and apoptosis. C. racemosa var. macrophysa showed the maximum effective activities with a lower dose of extract, about 130 ± 30 and 23 ± 1 μg ml EC dose for HeLa and Huh-7, respectively, followed by C. scalpelliformis, showing EC dose about 200 ± 10 and 140 ± 30 μg ml, respectively. Similarly, about 56% and 54% ROS inhibition were determined with Caulerpa spp. for HeLa and Huh-7 cells, respectively. Results indicated that tropical green seaweed Caulerpa spp. (C. racemosa var. macrophysa and C. scalpelliformis) have substantial potential of ROS inhibition. Further, it was observed that different cancer-linked marker proteins encoding genes were deferentially expressed with seaweed extracts in different cell lines. Overall, it is concluded that Caulerpa spp. are rich in antioxidant and anti-proliferative activities. Caulerpa spp. have potential to be explored further for cancer preventive properties or functional food or nutraceuticals applications.
海藻被认为是一种有前途的功能性食品，对人类食用是安全的，因为它们具有显著的健康益处。五种丰富的热带海藻，包括 Caulerpa racemosa var. macrophysa、Caulerpa scalpelliformis、Grateloupia indica、Sargassum linearifolium 和 Spatoglossum asperum，富含代谢物、酚类和类黄酮化合物，分析了其抗增殖和 ROS 抑制活性，包括与癌症相关的关键基因的转录表达和细胞凋亡。Caulerpa racemosa var. macrophysa 表现出最大的有效活性，其提取物的剂量较低，对 HeLa 和 Huh-7 的 EC 剂量分别约为 130±30 和 23±1μg/ml，其次是 C. scalpelliformis，其 EC 剂量分别约为 200±10 和 140±30μg/ml。同样，Caulerpa 属对 HeLa 和 Huh-7 细胞的 ROS 抑制率分别约为 56%和 54%。结果表明，热带绿海藻 Caulerpa 属（C. racemosa var. macrophysa 和 C. scalpelliformis）具有很强的 ROS 抑制潜力。此外，还观察到不同的癌症相关标记蛋白编码基因在不同的细胞系中以不同的方式表达。总的来说，结论是 Caulerpa 属富含抗氧化和抗增殖活性。Caulerpa 属具有进一步探索其预防癌症特性或作为功能性食品或营养保健品应用的潜力。
