氧化应激相关基因对纤维肌痛易感性的影响。

Influence of Oxidative Stress-Related Genes on Susceptibility to Fibromyalgia.

机构信息

Alma Rus, PhD, is Associate Professor, Department of Cell Biology Faculty of Sciences, University of Granada, Spain. Inmaculada Robles-Fernandez, PhD, is Research Associate, GENYO (Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research), Granada, Spain. Luis Javier Martinez-Gonzalez, PhD, is Senior Research Fellow, GENYO (Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research), Granada, Spain. Ramon Carmona, PhD, is Professor, Department of Cell Biology Faculty of Sciences, University of Granada, Spain. Maria Jesus Alvarez-Cubero, PhD, is Senior Research Fellow, GENYO (Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research), Granada, Spain. She is also Associate Professor, Department of Biochemistry and Molecular Biology III, Faculty of Medicine, University of Granada, Spain.

出版信息

Nurs Res. 2021 Jan/Feb;70(1):44-50. doi: 10.1097/NNR.0000000000000480.

DOI:10.1097/NNR.0000000000000480

PMID:32991532

Abstract

BACKGROUND

Fibromyalgia (FM) is a complex syndrome to diagnose and treat because of its unknown etiology. However, previous studies reported that patients with FM experience oxidative stress.

OBJECTIVES

In this study, we investigated single-nucleotide polymorphisms (SNPs) in genes encoding enzymes involved in oxidative stress (superoxide dismutase 1 [SOD1], catalase, and NADPH oxidase [CYBA]) in patients with FM and in healthy subjects, as well as the possible relation with demographic and clinical manifestations of FM.

METHODS

A total of 141 patients with FM and 73 healthy subjects participated in this case-control study. For DNA extraction, buccal swabs were collected from patients with FM, and a peripheral blood sample was extracted from controls. We analyzed SNPs in genes related to oxidative stress (rs10432782 in SOD1, rs1001179 in catalase, and rs4673 in CYBA) using TaqMan probes. In patients with FM, severity of FM, fatigue, and pain were assessed by Fibromyalgia Impact Questionnaire, Multidimensional Fatigue Inventory, and Visual Analogue Scale (VAS), respectively. Physical (PCS-12) and mental (MCS-12) health statuses were evaluated by the 12-Item Short-Form Health Survey.

RESULTS

The selected SNPs did not show significant differences between patients with FM and controls. The rs10432782 (SOD1) was associated with Fibromyalgia Impact Questionnaire scores in patients with FM, whereas the rs4673 (CYBA) was associated with the Multidimensional Fatigue Inventory score, MCS-12 score, and duration of the disease.

DISCUSSION

We have identified significant correlations between SOD1 and CYBA variants with clinical manifestations of FM. These results provide new insights into the pathogenesis of FM that could be useful for guiding future studies along the way to find the cause(s) of this syndrome.

摘要

背景

纤维肌痛（FM）是一种复杂的综合征，其病因不明，因此难以诊断和治疗。然而，先前的研究报告称，FM 患者存在氧化应激。

目的

本研究旨在调查 FM 患者和健康对照者编码参与氧化应激的酶的单核苷酸多态性（SNP）（超氧化物歧化酶 1[SOD1]、过氧化氢酶和 NADPH 氧化酶[CYBA]），并探讨其与 FM 的临床和人口统计学特征的可能关系。

方法

这项病例对照研究共纳入 141 例 FM 患者和 73 例健康对照者。采集 FM 患者的口腔拭子进行 DNA 提取，采集对照组的外周血样。我们使用 TaqMan 探针分析与氧化应激相关的基因（SOD1 的 rs10432782、过氧化氢酶的 rs1001179 和 CYBA 的 rs4673）的 SNP。采用纤维肌痛影响问卷（FIQ）、多维疲劳量表（MFI）和视觉模拟量表（VAS）评估 FM 患者的疾病严重程度、疲劳和疼痛，采用 12 项简短健康调查问卷（SF-12）评估患者的身体（PCS-12）和精神（MCS-12）健康状况。