Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC-Junta de Andalucía and Centro de Investigación Biomédica en Red de Enfermedades Raras, ISCIII, Sevilla, Spain.
PLoS One. 2012;7(4):e35677. doi: 10.1371/journal.pone.0035677. Epub 2012 Apr 19.
Fibromyalgia (FM) is a chronic pain syndrome with unknown etiology and a wide spectrum of symptoms such as allodynia, debilitating fatigue, joint stiffness and migraine. Recent studies have shown some evidences demonstrating that oxidative stress is associated to clinical symptoms in FM of fibromyalgia. We examined oxidative stress and bioenergetic status in blood mononuclear cells (BMCs) and its association to headache symptoms in FM patients. The effects of oral coenzyme Q(10) (CoQ(10)) supplementation on biochemical markers and clinical improvement were also evaluated.
We studied 20 FM patients and 15 healthy controls. Clinical parameters were evaluated using the Fibromyalgia Impact Questionnaire (FIQ), visual analogues scales (VAS), and the Headache Impact Test (HIT-6). Oxidative stress was determined by measuring CoQ(10), catalase and lipid peroxidation (LPO) levels in BMCs. Bioenergetic status was assessed by measuring ATP levels in BMCs.
We found decreased CoQ(10), catalase and ATP levels in BMCs from FM patients as compared to normal control (P < 0.05 and P < 0.001, respectively) We also found increased level of LPO in BMCs from FM patients as compared to normal control (P < 0.001). Significant negative correlations between CoQ(10) or catalase levels in BMCs and headache parameters were observed (r = -0.59, P < 0.05; r = -0.68, P < 0.05, respectively). Furthermore, LPO levels showed a significant positive correlation with HIT-6 (r = 0.33, P<0.05). Oral CoQ(10) supplementation restored biochemical parameters and induced a significant improvement in clinical and headache symptoms (P < 0.001).
The results of this study suggest a role for mitochondrial dysfunction and oxidative stress in the headache symptoms associated with FM. CoQ10 supplementation should be examined in a larger placebo controlled trial as a possible treatment in FM.
纤维肌痛（FM）是一种病因不明的慢性疼痛综合征，具有广泛的症状，如感觉过敏、使人虚弱的疲劳、关节僵硬和偏头痛。最近的研究表明，一些证据表明氧化应激与纤维肌痛的 FM 临床症状有关。我们检查了氧化应激和血液单核细胞（BMCs）中的生物能量状态及其与 FM 患者头痛症状的关系。还评估了口服辅酶 Q10（CoQ10）补充对生化标志物和临床改善的影响。
我们研究了 20 名 FM 患者和 15 名健康对照者。使用纤维肌痛影响问卷（FIQ）、视觉模拟量表（VAS）和头痛影响测试（HIT-6）评估临床参数。通过测量 BMCs 中的 CoQ10、过氧化氢酶和脂质过氧化（LPO）水平来确定氧化应激。通过测量 BMCs 中的 ATP 水平来评估生物能量状态。
我们发现 FM 患者 BMCs 中的 CoQ10、过氧化氢酶和 ATP 水平低于正常对照组（P<0.05 和 P<0.001）。我们还发现 FM 患者 BMCs 中的 LPO 水平高于正常对照组（P<0.001）。BMCs 中的 CoQ10 或过氧化氢酶水平与头痛参数之间存在显著负相关（r=-0.59，P<0.05；r=-0.68，P<0.05）。此外，LPO 水平与 HIT-6 呈显著正相关（r=0.33，P<0.05）。口服 CoQ10 补充恢复了生化参数，并显著改善了临床和头痛症状（P<0.001）。
这项研究的结果表明，线粒体功能障碍和氧化应激在与 FM 相关的头痛症状中起作用。CoQ10 补充剂应在更大的安慰剂对照试验中进行检查，作为 FM 的一种可能治疗方法。
